Application
Fig1: Western blot analysis of PKN1 on human PKN1 recombinant protein using anti-PKN1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PKN1 on HEK293 (1) and PKN1-hIgGFc transfected HEK293 (2) cell lysate using anti-PKN1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining PKN1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PKN1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with PKN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PKN1 [B1-10H] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DBK antibody PAK 1 antibody PAK-1 antibody PAK1 antibody PKC1 antibody PKN ALPHA antibody PKN antibody Pkn1 antibody PKN1_HUMAN antibody PRK1 antibody PRKCL1 antibody Protease activated kinase 1 antibody Protease-activated kinase 1 antibody Protein kinase C like 1 antibody Protein kinase C like PKN antibody Protein kinase C related kinase 1 antibody Protein kinase C-like 1 antibody Protein kinase C-like PKN antibody Protein kinase N1 antibody Protein kinase PKN alpha antibody Protein kinase PKN-alpha antibody Protein-kinase C-related kinase 1 antibody Serine threonine kinase N antibody Serine threonine protein kinase N antibody Serine-threonine protein kinase N antibody Serine/threonine protein kinase N1 antibody Serine/threonine-protein kinase N1 antibody |
| Product description | Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins act as molecular switches which are able to turn cytokinesis on and off. Although little is know about signaling downstream of Rho, several proteins have been implicated as Rho effectors. Protein kinase N (PKN) is a fatty acid-activated serine/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament protein. A second protein, rhophilin, exhibits 40% sequence identity with the amino terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron interacts specifically with active Rho and Rac1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | DBK antibody PAK 1 antibody PAK-1 antibody PAK1 antibody PKC1 antibody PKN ALPHA antibody PKN antibody Pkn1 antibody PKN1_HUMAN antibody PRK1 antibody PRKCL1 antibody Protease activated kinase 1 antibody Protease-activated kinase 1 antibody Protein kinase C like 1 antibody Protein kinase C like PKN antibody Protein kinase C related kinase 1 antibody Protein kinase C-like 1 antibody Protein kinase C-like PKN antibody Protein kinase N1 antibody Protein kinase PKN alpha antibody Protein kinase PKN-alpha antibody Protein-kinase C-related kinase 1 antibody Serine threonine kinase N antibody Serine threonine protein kinase N antibody Serine-threonine protein kinase N antibody Serine/threonine protein kinase N1 antibody Serine/threonine-protein kinase N1 antibody |
|---|---|
| Molecular Weight(MW) | 104 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Endosome. Cell membrane |
| SwissProt ID | Q16512 |
|---|
Application
Fig1: Western blot analysis of PKN1 on human PKN1 recombinant protein using anti-PKN1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PKN1 on HEK293 (1) and PKN1-hIgGFc transfected HEK293 (2) cell lysate using anti-PKN1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PKN1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PKN1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with PKN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PKN1 recombinant protein, PKN1-hIgGFc transfected HEK293 cell lysate, human colon cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
