Application
Fig1: Western blot analysis of SERPINA3 on human SERPINA3 recombinant protein using anti-SERPINA3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of SERPINA3 on HEK293 (1) and SERPINA3-hIgGFc transfected HEK293 (2) cell lysate using anti-SERPINA3 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of SERPINA3 on A549 cell lysate using anti-SERPINA3 antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-SERPINA3 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-SERPINA3 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of A549 cells with SERPINA3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | SERPINA3 [G3-C11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SERPINA3 antibody AACT antibody AACT_HUMAN antibody ACT antibody Alpha-1-antichymotrypsin His-Pro-less antibody Antichymotrypsin antibody Cell growth inhibiting gene 24/25 protein antibody Cell growth-inhibiting gene 24/25 protein antibody GIG24 antibody GIG25 antibody Growth inhibiting protein 24 antibody Growth inhibiting protein 25 antibody MGC88254 antibody Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody Serine (or cysteine) proteinase inhibitor clade A member 3 antibody Serine proteinase inhibitor clade A member 3 antibody Serpin A3 antibody Serpin family A member 3 antibody Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody SERPINA3 antibody |
| Product description | The serine proteinase inhibitors (serpins) are a superfamily of proteins with a diverse set of functions, including the control of blood coagulation, complement activation, programmed cell death and development. The most abundant serpins in human plasma are a 1-antitrypsin (AAT) and a 1-antichymotrypsin (AACT). AACT (also called A1AC and SERPINA3) is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of AAT, with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants; their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | SERPINA3 antibody
AACT antibody
AACT_HUMAN antibody
ACT antibody
Alpha-1-antichymotrypsin His-Pro-less antibody
Antichymotrypsin antibody
Cell growth inhibiting gene 24/25 protein antibody
Cell growth-inhibiting gene 24/25 protein antibody
GIG24 antibody
GIG25 antibody
Growth inhibiting protein 24 antibody
Growth inhibiting protein 25 antibody
MGC88254 antibody
Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody Serine (or cysteine) proteinase inhibitor clade A member 3 antibody Serine proteinase inhibitor clade A member 3 antibody Serpin A3 antibody Serpin family A member 3 antibody Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody SERPINA3 antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P01011 |
|---|
Application
Fig1: Western blot analysis of SERPINA3 on human SERPINA3 recombinant protein using anti-SERPINA3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SERPINA3 on HEK293 (1) and SERPINA3-hIgGFc transfected HEK293 (2) cell lysate using anti-SERPINA3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of SERPINA3 on A549 cell lysate using anti-SERPINA3 antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-SERPINA3 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-SERPINA3 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with SERPINA3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SERPINA3 recombinant protein, SERPINA3-hIgGFc transfected HEK293 cell lysate, A549 cell lysate, human endometrial cancer tissue, human bladder cancer tissue, A549. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
