Application
Fig1: Western blot analysis of RAP1GAP on human RAP1GAP recombinant protein using anti-RAP1GAP antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RAP1GAP on HEK293 (1) and RAP1GAP-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1GAP antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RAP1GAP [B6-G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA0474 antibody Rap1 GTPase activating protein 1 antibody RAP1 GTPase activating protein antibody Rap1 GTPase-activating protein 1 antibody RAP1GA1 antibody Rap1ga1 protein antibody Rap1GAP antibody Rap1GAP1 antibody RAP1GAPII antibody RAPGAP antibody RPGP1_HUMAN antibody |
| Product description | Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Gα z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, and also attenuates the ability of activated Gα z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Gα z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | KIAA0474 antibody Rap1 GTPase activating protein 1 antibody RAP1 GTPase activating protein antibody Rap1 GTPase-activating protein 1 antibody RAP1GA1 antibody Rap1ga1 protein antibody Rap1GAP antibody Rap1GAP1 antibody RAP1GAPII antibody RAPGAP antibody RPGP1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Golgi apparatus membrane |
| SwissProt ID | P47736 |
|---|
Application
Fig1: Western blot analysis of RAP1GAP on human RAP1GAP recombinant protein using anti-RAP1GAP antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAP1GAP on HEK293 (1) and RAP1GAP-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1GAP antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RAP1GAP recombinant protein, RAP1GAP-hIgGFc transfected HEK293 cell lysate, A431. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
