Application
Fig1: Western blot analysis of GRM3 on human GRM3 recombinant protein using anti-GRM3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of GRM3 on HEK293 (1) and GRM3-hIgGFc transfected HEK293 (2) cell lysate using anti-GRM3 antibody at 1/1,000 dilution.Application
Fig3: ICC staining GRM3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-GRM3 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of SH-SY5Y cells with GRM3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | GRM3 [G2-G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLUR3 antibody Glutamate metabotropic receptor 3 antibody Glutamate receptor metabotropic 3 antibody GPRC1C antibody GRM3 antibody GRM3_HUMAN antibody Metabotropic glutamate receptor 3 antibody MGlu3 antibody mGluR3 antibody |
| Product description | The mGluR proteins (metabotropic Glutamate Receptors) are members of the G-protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic Glutamate Receptors). The eight currently known mGluR proteins are mediated by two G-proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR1 and mGluR5 are mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight sub-types of mGluR have an activity mediated by a G-protein that inhibits adenylate cyclase activity. mGluR-3, which may interact with GRASP, acts as a receptor for glutamate. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | GLUR3 antibody Glutamate metabotropic receptor 3 antibody Glutamate receptor metabotropic 3 antibody GPRC1C antibody GRM3 antibody GRM3_HUMAN antibody Metabotropic glutamate receptor 3 antibody MGlu3 antibody mGluR3 antibody |
|---|---|
| Molecular Weight(MW) | 99 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | Q14832 |
|---|
Application
Fig1: Western blot analysis of GRM3 on human GRM3 recombinant protein using anti-GRM3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of GRM3 on HEK293 (1) and GRM3-hIgGFc transfected HEK293 (2) cell lysate using anti-GRM3 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining GRM3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-GRM3 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of SH-SY5Y cells with GRM3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human GRM3 recombinant protein, GRM3-hIgGFc transfected HEK293 cell lysate, Hela, human cervical cancer tissue, SH-SY5Y. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
