Application
Fig1: Western blot analysis of SNAI2 on human SNAI2 recombinant protein using anti-SNAI2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of SNAI2 on HEK293 (1) and SNAI2-hIgGFc transfected HEK293 (2) cell lysate using anti-SNAI2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of SNAI2 on MCF-7 cell lysate using anti-SNAI2 antibody at 1/1,000 dilution.Application
Fig4: ICC staining SNAI2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded ?human cervical cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of MCF-7 cells with SNAI2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | SNAI2 [D5-B6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC10182 antibody Neural crest transcription factor Slug antibody Protein snail homolog 2 antibody Slug (chicken homolog) zinc finger protein antibody Slug homolog zinc finger protein antibody Slug zinc finger protein antibody SLUGH antibody SLUGH 1 antibody SLUGH1 antibody SNAI 2 antibody Snai2 antibody SNAI2_HUMAN antibody Snail 2 antibody Snail homolog 2 antibody Snail2 antibody WS 2D antibody WS2D antibody Zinc finger protein SLUG antibody Zinc finger protein SNAI2 antibody |
| Product description | The SNAIL family of developmental regulatory proteins is a group of widely conserved zinc-finger proteins that regulate transcription and include the mammalian proteins SLUG, SNAI 1 (the human homolog of Drosophila SNAIL) and Smuc. SNAI 1 and SLUG are expressed in placenta and in adult heart, liver, and skeletal muscle. SNAI 1, and the corresponding mouse homolog Sna, each contain three classic zinc fingers and one atypical zinc finger, while SLUG contains five zinc finger regions and a transcriptional repression domain at the amino terminus, which enables SLUG to act as a negative regulator of gene expression. SLUG is implicated in the generation and migration of neural crest cells in human embryos and also contributes to limb bud development. In addition, SLUG also constitutes a cellular anti-apoptotic transcription factor that effectively prevents apoptosis in murine pro-B cells deprived of IL-3. The SNAIL-related gene from murine skeletal muscle cells, Smuc, is highly expressed in skeletal muscle and thymus and can, likewise, repress gene transcription. Smuc preferentially associates with CAGGTG and CACCTG E-box motifs (CANNTG) on DNA and involves the five putative DNA-binding zinc finger domains at the C-terminal region of Smuc. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | MGC10182 antibody Neural crest transcription factor Slug antibody Protein snail homolog 2 antibody Slug (chicken homolog) zinc finger protein antibody Slug homolog zinc finger protein antibody Slug zinc finger protein antibody SLUGH antibody SLUGH 1 antibody SLUGH1 antibody SNAI 2 antibody Snai2 antibody SNAI2_HUMAN antibody Snail 2 antibody Snail homolog 2 antibody Snail2 antibody WS 2D antibody WS2D antibody Zinc finger protein SLUG antibody Zinc finger protein SNAI2 antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Nucleus,cytoplsm |
| SwissProt ID | O43623 |
|---|
Application
Fig1: Western blot analysis of SNAI2 on human SNAI2 recombinant protein using anti-SNAI2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SNAI2 on HEK293 (1) and SNAI2-hIgGFc transfected HEK293 (2) cell lysate using anti-SNAI2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of SNAI2 on MCF-7 cell lysate using anti-SNAI2 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining SNAI2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded ?human cervical cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of MCF-7 cells with SNAI2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SNAI2 recombinant protein, SNAI2-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7, human colon cancer tissue, human cervical cancer tissue |
|---|---|
| Application Notes | WB:1:500-1:2000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
