Application
Fig1: Western blot analysis of NADPH oxidase 4 on human NADPH oxidase 4 recombinant protein using anti- NADPH oxidase 4 antibody at 1/1,000.Application
Fig2: Western blot analysis of NADPH oxidase 4 on HEK293 (1) and NADPH oxidase 4-hIgGFc transfected HEK293 (2) cell lysate using anti- NADPH oxidase 4 antibody at 1/1,000 dilution.Application
Fig3: ICC staining NADPH oxidase 4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- NADPH oxidase 4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- NADPH oxidase 4 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with NADPH oxidase 4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | NOX4 [G11-H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kidney oxidase 1 antibody Kidney oxidase-1 antibody Kidney superoxide producing NADPH oxidase antibody Kidney superoxide-producing NADPH oxidase antibody KOX 1 antibody KOX antibody Kox-1 antibody KOX1 antibody NADPH antibody NADPH oxidase 4 antibody Nox4 antibody NOX4_HUMAN antibody Renal NAD(P)H oxidase antibody Renal NAD(P)H-oxidase antibody RENOX antibody |
| Product description | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Kidney oxidase 1 antibody Kidney oxidase-1 antibody Kidney superoxide producing NADPH oxidase antibody Kidney superoxide-producing NADPH oxidase antibody KOX 1 antibody KOX antibody Kox-1 antibody KOX1 antibody NADPH antibody NADPH oxidase 4 antibody Nox4 antibody NOX4_HUMAN antibody Renal NAD(P)H oxidase antibody Renal NAD(P)H-oxidase antibody RENOX antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. Cell membrane |
| SwissProt ID | Q9NPH5 |
|---|
Application
Fig1: Western blot analysis of NADPH oxidase 4 on human NADPH oxidase 4 recombinant protein using anti- NADPH oxidase 4 antibody at 1/1,000.
Application
Fig2: Western blot analysis of NADPH oxidase 4 on HEK293 (1) and NADPH oxidase 4-hIgGFc transfected HEK293 (2) cell lysate using anti- NADPH oxidase 4 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining NADPH oxidase 4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- NADPH oxidase 4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- NADPH oxidase 4 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with NADPH oxidase 4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human NOX4 recombinant protein, NOX4-hIgGFc transfected HEK293 cell lysate, Hela, human cervical cancer tissue, human bladder cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
