Application
Fig1: Western blot analysis of WTAP on human WTAP recombinant protein using anti-WTAP antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of WTAP on HEK293 (1) and WTAP-hIgGFc transfected HEK293 (2) cell lysate using anti-WTAP antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of WTAP on different cell lysate using anti-WTAP antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: Hela Line3: K562 Line4: Hek293 Line5: A549 Line6: HepG2 Line7: Jurkat Line8: Cos7Application
Fig4: ICC staining WTAP (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of A549 cells with WTAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | WTAP [B6-B6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686F20131 antibody Female-lethal(2)D homolog antibody FL2D_HUMAN antibody hFL(2)D antibody KIAA0105 antibody MGC3925 antibody Mum2 antibody OTTHUMP00000017522 antibody OTTHUMP00000017523 antibody PNAS 132 antibody PNAS-132 antibody PNAS132 antibody Pre mRNA splicing regulator WTAP antibody Pre-mRNA-splicing regulator WTAP antibody Putative pre mRNA splicing regulator female lethal 2D homolog antibody Putative pre mRNA splicing regulator female lethal(2D) antibody Putative pre-mRNA splicing regulator female lethal(2D) homolog antibody putative pre-mRNA splicing regulator female-lethal(2D) antibody Wilms tumor 1 associated protein antibody Wilms tumor 1 associating protein antibody Wilms tumor 1-associating protein antibody Wilms' tumor 1 associating protein antibody Wilms' tumour 1-associating protein antibody WT1 associated protein antibody WT1-associated protein antibody WT1-associating protein antibody wtap antibody |
| Product description | Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles where it regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | DKFZp686F20131 antibody Female-lethal(2)D homolog antibody FL2D_HUMAN antibody hFL(2)D antibody KIAA0105 antibody MGC3925 antibody Mum2 antibody OTTHUMP00000017522 antibody OTTHUMP00000017523 antibody PNAS 132 antibody PNAS-132 antibody PNAS132 antibody Pre mRNA splicing regulator WTAP antibody Pre-mRNA-splicing regulator WTAP antibody Putative pre mRNA splicing regulator female lethal 2D homolog antibody Putative pre mRNA splicing regulator female lethal(2D) antibody Putative pre-mRNA splicing regulator female lethal(2D) homolog antibody putative pre-mRNA splicing regulator female-lethal(2D) antibody Wilms tumor 1 associated protein antibody Wilms tumor 1 associating protein antibody Wilms tumor 1-associating protein antibody Wilms' tumor 1 associating protein antibody Wilms' tumour 1-associating protein antibody WT1 associated protein antibody WT1-associated protein antibody WT1-associating protein antibody wtap antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Nucleus, nucleolus |
| SwissProt ID | Q15007 |
|---|
Application
Fig1: Western blot analysis of WTAP on human WTAP recombinant protein using anti-WTAP antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of WTAP on HEK293 (1) and WTAP-hIgGFc transfected HEK293 (2) cell lysate using anti-WTAP antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of WTAP on different cell lysate using anti-WTAP antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: Hela Line3: K562 Line4: Hek293 Line5: A549 Line6: HepG2 Line7: Jurkat Line8: Cos7
Application
Fig4: ICC staining WTAP (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of A549 cells with WTAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human WTAP recombinant protein, WTAP-hIgGFc transfected HEK293 cell lysate, MCF-7, Hela, K562, Hek293, A549, HepG2, Jurkat, Cos7, human cervical cancer tissue, human endometrial cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
