Application
Fig1: Western blot analysis of DDX39B on human DDX39B recombinant protein using anti- UAP56 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of DDX39B on HEK293 (1) and DDX39B-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX39B antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of DDX39B on different cells lysates using anti-DDX39B antibody at 1/1,000 dilution.Positive control：Line1: HEK293 Line2: JurkatLine3: MCF-7 Line4: A431Line5: NIH/3T3 Line6: JurkatLine7: K562 Line8: HepG2Application
Fig4: ICC staining DDX39B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-DDX39B antibody. Counter stained with hematoxylin.| Product Name | DDX39B [B2-A2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 0610030D10Rik antibody 4F2-LC6 antibody 56 kDa U2AF65-associated protein antibody AI428441 antibody ATP-dependent RNA helicase p47 antibody B(0,+)-type amino acid transporter 1 antibody BAT1 antibody Bat1a antibody D17H6S81E antibody D17H6S81E-1 antibody D6S81E antibody D6S81Eh antibody DDX39B antibody DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B antibody DEAD box protein UAP56 antibody DX39B_HUMAN antibody EC 3.6.1.- antibody Glycoprotein-associated amino acid transporter b0,+AT1 antibody HLA-B-associated transcript 1 protein antibody HLA-B-associated transcript 1A antibody HLA-B-associated transcript-1 antibody MGC127051 antibody MGC19235 antibody MGC38799 antibody nuclear RNA helicase (DEAD family) antibody OTTHUMP00000029229 antibody OTTHUMP00000165889 antibody OTTHUMP00000165890 antibody p47 antibody Solute carrier family 7 member 9 antibody Spliceosome RNA helicase BAT1 antibody Spliceosome RNA helicase DDX39B antibody U2AF65-associayed protein, 56-KD antibody UAP56 antibody |
| Product description | BAT1 (spliceosome RNA helicase BAT1), also known as DEAD-box protein UAP56 (56 kDa U2AF65-associated protein), HLA-B associated transcript-1 or ATP-dependent RNA helicase p47, is a member of the DECD subfamily of DEAD-box helicases. Important for mRNA splicing and nuclear export, BAT1 interacts with the mRNA export factor ALY and also functions as a splicing factor, mediating the first ATP-dependent step of spliceosome assembly. BAT1 associates with transcription elongation factor THO proteins, forming the TREX complex, and also interacts with splicing machinery to form the exon junction complex. Due to alternative splicing events, BAT1 exists in two isoforms. DDX39 (DEAD box protein 39), like BAT1, is a member of the DEAD-box family of helicases. Localized to the nucleus and expressed in lung, brain, kidney, splenn, thymus and salivary gland, DDX39 functions in a similar manner to BAT1 and is involved in pre-mRNA splicing and mRNA export out of the nucleus. DDX39 expression is upregulated in lung squamous cell carcinoma, suggesting a role for DDX39 in tumorigenesis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 0610030D10Rik antibody
4F2-LC6 antibody
56 kDa U2AF65-associated protein antibody
AI428441 antibody
ATP-dependent RNA helicase p47 antibody
B(0 +)-type amino acid transporter 1 antibody BAT1 antibody Bat1a antibody D17H6S81E antibody D17H6S81E-1 antibody D6S81E antibody D6S81Eh antibody DDX39B antibody DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B antibody DEAD box protein UAP56 antibody DX39B_HUMAN antibody EC 3.6.1.- antibody Glycoprotein-associated amino acid transporter b0 +AT1 antibody HLA-B-associated transcript 1 protein antibody HLA-B-associated transcript 1A antibody HLA-B-associated transcript-1 antibody MGC127051 antibody MGC19235 antibody MGC38799 antibody nuclear RNA helicase (DEAD family) antibody OTTHUMP00000029229 antibody OTTHUMP00000165889 antibody OTTHUMP00000165890 antibody p47 antibody Solute carrier family 7 member 9 antibody Spliceosome RNA helicase BAT1 antibody Spliceosome RNA helicase DDX39B antibody U2AF65-associayed protein 56-KD antibody UAP56 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Nucleus. Nucleus speckle |
Application
Fig1: Western blot analysis of DDX39B on human DDX39B recombinant protein using anti- UAP56 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DDX39B on HEK293 (1) and DDX39B-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX39B antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of DDX39B on different cells lysates using anti-DDX39B antibody at 1/1,000 dilution.Positive control：Line1: HEK293 Line2: JurkatLine3: MCF-7 Line4: A431Line5: NIH/3T3 Line6: JurkatLine7: K562 Line8: HepG2
Application
Fig4: ICC staining DDX39B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-DDX39B antibody. Counter stained with hematoxylin.| Positive Control | Human DDX39B recombinant protein, DDX39B-hIgGFc transfected HEK293 cell lysate, HEK293, Jurkat, MCF-7, A431, NIH/3T3, K562, HepG2, human cervical cancer tissues. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
