Application
Fig1: Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of EPN1 on HEK293 (1) and EPN1-hIgGFc transfected HEK293 (2) cell lysate using anti-EPN1 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of A431 cells with EPN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | EPN1 [F9-F7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EH domain binding mitotic phosphoprotein antibody EH domain-binding mitotic phosphoprotein antibody EPN 1 antibody Epn1 antibody EPN1_HUMAN antibody EPS 15 interacting protein 1 antibody EPS-15-interacting protein 1 antibody Epsin 1 antibody Epsin-1 antibody |
| Product description | Epsin 1 (EPN1) is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the 伪 subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both epsin and Eps15, like other cytosolic components of the synaptic vesicle endocytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | EH domain binding mitotic phosphoprotein antibody EH domain-binding mitotic phosphoprotein antibody EPN 1 antibody Epn1 antibody EPN1_HUMAN antibody EPS 15 interacting protein 1 antibody EPS-15-interacting protein 1 antibody Epsin 1 antibody Epsin-1 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cytoplasm. Cell membrane. Nucleus |
| SwissProt ID | Q9Y6I3 |
|---|
Application
Fig1: Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of EPN1 on HEK293 (1) and EPN1-hIgGFc transfected HEK293 (2) cell lysate using anti-EPN1 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of A431 cells with EPN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human EPN1 recombinant protein, EPN1-hIgGFc transfected HEK293 cell lysate, A431. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
