Application
Fig1: Western blot analysis of Aurora A on human Aurora A recombinant protein using anti- Aurora A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Aurora A on HEK293 (1) and Aurora A -hIgGFc transfected HEK293 (2) cell lysate using anti- Aurora A antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of Aurora A on different cell lysate using anti- Aurora A antibody at 1/1,000 dilution. Positive control： Line1: HEK293 Line2: MCF-7 Line3: HelaApplication
Fig4: ICC staining Aurora A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining Aurora A (green) and Actin filaments (red) in SMMC-7721 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Aurora A antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- Aurora A antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with Aurora A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | AURKA [A4-8D] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIK antibody ARK-1 antibody ARK1 antibody AURA antibody Aurka antibody Aurora 2 antibody Aurora A antibody Aurora kinase A antibody Aurora-related kinase 1 antibody Aurora/IPL1 like kinase antibody Aurora/IPL1-related kinase 1 antibody AURORA2 antibody Breast tumor-amplified kinase antibody BTAK antibody hARK1 antibody IAK antibody IPL1 related kinase antibody MGC34538 antibody OTTHUMP00000031340 antibody OTTHUMP00000031341 antibody OTTHUMP00000031342 antibody OTTHUMP00000031343 antibody OTTHUMP00000031344 antibody OTTHUMP00000031345 antibody OTTHUMP00000166071 antibody OTTHUMP00000166072 antibody PPP1R47 antibody Protein phosphatase 1, regulatory subunit 47 antibody Serine/threonine kinase 15 antibody Serine/threonine kinase 6 antibody Serine/threonine-protein kinase 15 antibody Serine/threonine-protein kinase 6 antibody Serine/threonine-protein kinase aurora-A antibody STK15 antibody STK6 antibody STK6_HUMAN antibody STK7 antibody |
| Product description | Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/threonine kinases that regulate centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1) and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AIK antibody
ARK-1 antibody
ARK1 antibody
AURA antibody
Aurka antibody
Aurora 2 antibody
Aurora A antibody
Aurora kinase A antibody
Aurora-related kinase 1 antibody
Aurora/IPL1 like kinase antibody
Aurora/IPL1-related kinase 1 antibody
AURORA2 antibody
Breast tumor-amplified kinase antibody
BTAK antibody
hARK1 antibody
IAK antibody
IPL1 related kinase antibody
MGC34538 antibody
OTTHUMP00000031340 antibody
OTTHUMP00000031341 antibody
OTTHUMP00000031342 antibody
OTTHUMP00000031343 antibody
OTTHUMP00000031344 antibody
OTTHUMP00000031345 antibody
OTTHUMP00000166071 antibody
OTTHUMP00000166072 antibody
PPP1R47 antibody
Protein phosphatase 1 regulatory subunit 47 antibody Serine/threonine kinase 15 antibody Serine/threonine kinase 6 antibody Serine/threonine-protein kinase 15 antibody Serine/threonine-protein kinase 6 antibody Serine/threonine-protein kinase aurora-A antibody STK15 antibody STK6 antibody STK6_HUMAN antibody STK7 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cell membrane. Endosome membrane. Lysosome membrane |
Application
Fig1: Western blot analysis of Aurora A on human Aurora A recombinant protein using anti- Aurora A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Aurora A on HEK293 (1) and Aurora A -hIgGFc transfected HEK293 (2) cell lysate using anti- Aurora A antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of Aurora A on different cell lysate using anti- Aurora A antibody at 1/1,000 dilution. Positive control： Line1: HEK293 Line2: MCF-7 Line3: Hela
Application
Fig4: ICC staining Aurora A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining Aurora A (green) and Actin filaments (red) in SMMC-7721 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Aurora A antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- Aurora A antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with Aurora A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Aurora A recombinant protein, Aurora A -hIgGFc transfected HEK293 cell lysate, HEK293, MCF-7, Hela cell lysates, Hela, SMMC-7721, human cervical cancer tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
