Application
Fig1: Western blot analysis of Emerin on human Emerin recombinant protein using anti- Emerin antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Emerin on HEK293 (1) and Emerin -hIgGFc transfected HEK293 (2) cell lysate using anti- Emerin antibody at 1/1,000 dilution.Application
Fig3: ICC staining Emerin (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- Emerin antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of A549 cells with Emerin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).Application
Fig6: Flow cytometric analysis of K562 cells with Emerin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | EMD [A8-F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EDMD antibody Emd antibody EMD_HUMAN antibody Emerin antibody Emery Dreifuss muscular dystrophy antibody STA antibody |
| Product description | Emerin is believed to be a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreyfuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Research has demonstrated that the lack of emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherentes in the heart. This may account for conduction defects in patients with EDMD. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | EDMD antibody Emd antibody EMD_HUMAN antibody Emerin antibody Emery Dreifuss muscular dystrophy antibody STA antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa |
| Cellular Localization | Nucleus inner membrane. Nucleus outer membrane |
| SwissProt ID | P50402 |
|---|
Application
Fig1: Western blot analysis of Emerin on human Emerin recombinant protein using anti- Emerin antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Emerin on HEK293 (1) and Emerin -hIgGFc transfected HEK293 (2) cell lysate using anti- Emerin antibody at 1/1,000 dilution.
Application
Fig3: ICC staining Emerin (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- Emerin antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of A549 cells with Emerin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Application
Fig6: Flow cytometric analysis of K562 cells with Emerin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Emerin recombinant protein, Emerin -hIgGFc transfected HEK293 cell lysate, Hela, human ovarian cancer tissue, A549, K562. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
