Application
Fig1: Western blot analysis of CHRNA5 on human CHRNA5 recombinant protein using anti-CHRNA5 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CHRNA5 on HEK293 (1) and CHRNA5-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA5 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CHRNA5 on different cell lysate using anti-CHRNA5 antibody at 1/1,000 dilution. Positive control： Line1: membrane protein lysate of C6 Line2: membrane protein lysate of SK-N-SH Line3: membrane protein lysate of C6 Line4: C6 cell lysateApplication
Fig4: Flow cytometric analysis of SK-N-SH cells with CHRNA5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CHRNA5 [D10-D2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody ACHA5_HUMAN antibody AChR antibody Cholinergic receptor neuronal nicotinic alpha polypeptide 5 antibody Cholinergic receptor nicotinic alpha 5 antibody Cholinergic receptor nicotinic alpha polypeptide 5 antibody CHRNA 5 antibody CHRNA5 antibody LNCR2 antibody NACHRA 5 antibody NACHRA5 antibody Neuronal acetylcholine receptor subunit alpha 5 antibody Neuronal acetylcholine receptor subunit alpha-5 antibody Neuronal nicotinic acetylcholine receptor alpha 5 subunit antibody Neuronal nicotinic acetylcholine receptor, alpha5 subunit antibody OTTHUMP00000185019 antibody |
| Product description | Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChRα5, also known as LNCR2 or CHRNA5 (cholinergic receptor, nicotinic, alpha 5), is a 468 amino acid multi-pass membrane protein belonging to the ligand-gated ionic channel family and is involved in the mediation of fast signal transmission at synapses. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody
ACHA5_HUMAN antibody
AChR antibody
Cholinergic receptor neuronal nicotinic alpha polypeptide 5 antibody
Cholinergic receptor nicotinic alpha 5 antibody
Cholinergic receptor nicotinic alpha polypeptide 5 antibody
CHRNA 5 antibody
CHRNA5 antibody
LNCR2 antibody
NACHRA 5 antibody
NACHRA5 antibody
Neuronal acetylcholine receptor subunit alpha 5 antibody
Neuronal acetylcholine receptor subunit alpha-5 antibody
Neuronal nicotinic acetylcholine receptor alpha 5 subunit antibody
Neuronal nicotinic acetylcholine receptor alpha5 subunit antibody OTTHUMP00000185019 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Cell junction, synapse, postsynaptic cell membrane. Cell membrane |
Application
Fig1: Western blot analysis of CHRNA5 on human CHRNA5 recombinant protein using anti-CHRNA5 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CHRNA5 on HEK293 (1) and CHRNA5-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA5 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CHRNA5 on different cell lysate using anti-CHRNA5 antibody at 1/1,000 dilution. Positive control： Line1: membrane protein lysate of C6 Line2: membrane protein lysate of SK-N-SH Line3: membrane protein lysate of C6 Line4: C6 cell lysate
Application
Fig4: Flow cytometric analysis of SK-N-SH cells with CHRNA5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CHRNA5 recombinant protein, CHRNA5-hIgGFc transfected HEK293 cell lysate, membrane protein lysate of C6, membrane protein lysate of SK-N-SH, C6 cell lysate, SK-N-SH. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
