Application
Fig1: Western blot analysis of Calretinin on human Calretinin recombinant protein using anti- Calretinin antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Calretinin on HEK293 (1) and Calretinin -hIgGFc transfected HEK293 (2) cell lysate using anti- Calretinin antibody at 1/1,000 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- Calretinin antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of A549 cells with Calretinin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CALB2 [H1-F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 29 kDa calbindin antibody CAB 29 antibody CAB29 antibody CAL 2 antibody CAL2 antibody CALB 2 antibody CALB2 antibody CALB2_HUMAN antibody Calbindin 2 29kDa antibody Calbindin 2 antibody Calbindin D29K antibody Calbindin2 antibody Calretinin antibody CR antibody |
| Product description | Calbindin D28K and Calretinin (also designated CR or 29 kDa Calbindin) are two closely related intracellular calcium-binding proteins belonging to the Troponin-C superfamily. Initially isolated from chick retina, Calretinin shares 58% identical residues with human Calbindin D28K. Calretinin is expressed in the brain and is particularly abundant in auditory neurons with precisely timed discharges. Neurons in the nucleus accumbens containing Calretinin all possess nuclear indentations. Calretinin-immunoreactive boutons form asymmetrical and symmetrical synaptic specializations on spines, dendrites and somata. The symmetrical synaptic specializations have medium-sized spiny neurons and contact other Calretinin-immunoreactive somata. Calretinin is widely used as a immunocytochemical marker for mesothelioma. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 29 kDa calbindin antibody CAB 29 antibody CAB29 antibody CAL 2 antibody CAL2 antibody CALB 2 antibody CALB2 antibody CALB2_HUMAN antibody Calbindin 2 29kDa antibody Calbindin 2 antibody Calbindin D29K antibody Calbindin2 antibody Calretinin antibody CR antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytosol |
| SwissProt ID | P22676 |
|---|
Application
Fig1: Western blot analysis of Calretinin on human Calretinin recombinant protein using anti- Calretinin antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Calretinin on HEK293 (1) and Calretinin -hIgGFc transfected HEK293 (2) cell lysate using anti- Calretinin antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- Calretinin antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of A549 cells with Calretinin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human calretinin recombinant protein, Calretinin-hIgGFc transfected HEK293 cell lysate, human brain tissue, A549. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
