Application
Fig1: Western blot analysis of CBX1 on human CBX1 recombinant protein using anti-CBX1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CBX1 on HEK293 (1) and CBX1-hIgGFc transfected HEK293 (2) cell lysate using anti-CBX1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining CBX1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CBX1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CBX1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CBX1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CBX1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CBX1 [B8-D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBX 1 antibody CBX antibody Cbx1 antibody CBX1_HUMAN antibody Chromobox 1 antibody Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody chromobox homolog 1 antibody Chromobox protein homolog 1 antibody Drosophila HP1 beta antibody Heterochromatin protein 1 beta antibody Heterochromatin protein 1 homolog beta antibody Heterochromatin protein p25 antibody heterochromatin protein p25 beta antibody HP1 beta antibody HP1 beta homolog antibody HP1 beta homolog Drosophila antibody HP1, Drosophila. homolog of, beta antibody HP1beta antibody HP1Hs beta antibody HP1Hsbeta antibody Human heterochromatin protein p25 mRNA complete cds antibody M31 antibody MOD 1 antibody MOD1 antibody Modifier 1 protein antibody p25beta antibody |
| Product description | Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of Histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CBX 1 antibody
CBX antibody
Cbx1 antibody
CBX1_HUMAN antibody
Chromobox 1 antibody
Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody
chromobox homolog 1 antibody
Chromobox protein homolog 1 antibody
Drosophila HP1 beta antibody
Heterochromatin protein 1 beta antibody
Heterochromatin protein 1 homolog beta antibody
Heterochromatin protein p25 antibody
heterochromatin protein p25 beta antibody
HP1 beta antibody
HP1 beta homolog antibody
HP1 beta homolog Drosophila antibody
HP1 Drosophila. homolog of beta antibody HP1beta antibody HP1Hs beta antibody HP1Hsbeta antibody Human heterochromatin protein p25 mRNA complete cds antibody M31 antibody MOD 1 antibody MOD1 antibody Modifier 1 protein antibody p25beta antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P83916 |
|---|
Application
Fig1: Western blot analysis of CBX1 on human CBX1 recombinant protein using anti-CBX1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CBX1 on HEK293 (1) and CBX1-hIgGFc transfected HEK293 (2) cell lysate using anti-CBX1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining CBX1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CBX1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CBX1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CBX1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CBX1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CBX1 recombinant protein, CBX1-hIgGFc transfected HEK293 cell lysate, Hela, human cervical cancer tissue, human ovarian cancer tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
