Application
Fig1: Western blot analysis of EZR on human EZR recombinant protein using anti-EZR antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of EZR on HEK293 (1) and EZR-hIgGFc transfected HEK293 (2) cell lysate using anti-EZR antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of EZR on different cell lysate using anti-EZR antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: Hela Line3: A431 Line4: Hek293 Line5: SK-N-SH Line6: Jurkat Line7: HepG2 Line8: NIH/3T3 Line9: Cos7Application
Fig4: ICC staining EZR (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).Application
Fig6: Flow cytometric analysis of MCF-7 cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | EZR [F1-9A] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Villin 2 ezrin antibody CVIL antibody CVL antibody Cytovillin 2 antibody Cytovillin antibody DKFZp762H157 antibody Epididymis secretory protein Li 105 antibody EZR antibody EZRI_HUMAN antibody Ezrin antibody FLJ26216 antibody HEL S 105 antibody MGC1584 antibody p81 antibody VIL 2 antibody VIL2 antibody Villin 2 (ezrin) antibody Villin 2 antibody Villin-2 antibody Villin2 antibody |
| Product description | Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins (2-5). Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | Villin 2 ezrin antibody CVIL antibody CVL antibody Cytovillin 2 antibody Cytovillin antibody DKFZp762H157 antibody Epididymis secretory protein Li 105 antibody EZR antibody EZRI_HUMAN antibody Ezrin antibody FLJ26216 antibody HEL S 105 antibody MGC1584 antibody p81 antibody VIL 2 antibody VIL2 antibody Villin 2 (ezrin) antibody Villin 2 antibody Villin-2 antibody Villin2 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Apical cell membrane. Cell projection. |
Application
Fig1: Western blot analysis of EZR on human EZR recombinant protein using anti-EZR antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of EZR on HEK293 (1) and EZR-hIgGFc transfected HEK293 (2) cell lysate using anti-EZR antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of EZR on different cell lysate using anti-EZR antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: Hela Line3: A431 Line4: Hek293 Line5: SK-N-SH Line6: Jurkat Line7: HepG2 Line8: NIH/3T3 Line9: Cos7
Application
Fig4: ICC staining EZR (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Application
Fig6: Flow cytometric analysis of MCF-7 cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human EZR recombinant protein, EZR-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7, A431, Hek293, SK-N-SH, Jurkat, HepG2, NIH/3T3, Cos7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
