Application
Fig1: Western blot analysis of MECP2 on human MECP2 recombinant protein using anti-MECP2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of MECP2 on HEK293 (1) and MECP2-hIgGFc transfected HEK293 (2) cell lysate using anti-MECP2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of MECP2 on A431 (1) and MCF-7 (2) cell lysate using anti-MECP2 antibody at 1/1,000 dilution.Application
Fig4: ICC staining MECP2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% TritonApplication
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-MECP2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-MECP2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with MECP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | MECP2 [5B-9H] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AUTSX 3 antibody AUTSX3 antibody DKFZp686A24160 antibody Mbd 5 antibody Mbd5 antibody MECP 2 antibody MeCP 2 protein antibody MeCP-2 protein antibody Mecp2 antibody MECP2_HUMAN antibody Methyl CpG binding protein 2 (Rett syndrome) antibody Methyl CpG binding protein 2 antibody Methyl-CpG-binding protein 2 antibody MRX 16 antibody MRX 79 antibody MRX16 antibody MRX79 antibody MRXS 13 antibody MRXS13 antibody MRXSL antibody PPMX antibody RS antibody RTS antibody RTT antibody WBP 10 antibody WBP10 antibody |
| Product description | Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5' cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. MBD1 binds in a context specific manner to methyl-CpG rich domains and, in turn, mediates the transcriptional inhibition that is commonly observed with DNA methylation. Similarly, MBD2 inhibits transcription of methylated genes by associating with histone deacetylase (HDAC1) within the MeCP1 repressor complex. In addition, MBD4, which is also designated MED1, associates with the mismatch repair protein MLH1 and preferentially binds to methylated cytosine residues in mismatched base pairs. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AUTSX 3 antibody AUTSX3 antibody DKFZp686A24160 antibody Mbd 5 antibody Mbd5 antibody MECP 2 antibody MeCP 2 protein antibody MeCP-2 protein antibody Mecp2 antibody MECP2_HUMAN antibody Methyl CpG binding protein 2 (Rett syndrome) antibody Methyl CpG binding protein 2 antibody Methyl-CpG-binding protein 2 antibody MRX 16 antibody MRX 79 antibody MRX16 antibody MRX79 antibody MRXS 13 antibody MRXS13 antibody MRXSL antibody PPMX antibody RS antibody RTS antibody RTT antibody WBP 10 antibody WBP10 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P51608 |
|---|
Application
Fig1: Western blot analysis of MECP2 on human MECP2 recombinant protein using anti-MECP2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MECP2 on HEK293 (1) and MECP2-hIgGFc transfected HEK293 (2) cell lysate using anti-MECP2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of MECP2 on A431 (1) and MCF-7 (2) cell lysate using anti-MECP2 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining MECP2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-MECP2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-MECP2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with MECP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human MECP2 recombinant protein, MECP2-hIgGFc transfected HEK293 cell lysate, Hela, A431, MCF-7, human ovarian cancer tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
