Application
Fig1: Western blot analysis of PRDM1 on human PRDM1 recombinant protein using anti-PRDM1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PRDM1 on HEK293 (1) and PRDM1-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining PRDM1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Raji cells with PRDM1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PRDM1 [B6-F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B Lymphocyte Induced Maturation Protein 1 antibody Beta interferon gene positive regulatory domain I binding factor antibody Beta-interferon gene positive regulatory domain I-binding factor antibody BLIMP-1 antibody BLIMP1 antibody Positive Regulatory Domain I Binding Factor 1 antibody Positive regulatory domain I-binding factor 1 antibody PR Domain Containing 1 antibody PR domain containing 1 with ZNF domain antibody PR domain containing 1 with ZNF domain isoform 2 antibody PR domain containing protein 1 antibody PR domain zinc finger protein 1 antibody PR domain-containing protein 1 antibody PRDI BF1 antibody PRDI binding factor 1 antibody PRDI-BF1 antibody PRDI-binding factor 1 antibody PRDM 1 antibody Prdm1 antibody PRDM1_HUMAN antibody |
| Product description | The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:200-1:1,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | B Lymphocyte Induced Maturation Protein 1 antibody Beta interferon gene positive regulatory domain I binding factor antibody Beta-interferon gene positive regulatory domain I-binding factor antibody BLIMP-1 antibody BLIMP1 antibody Positive Regulatory Domain I Binding Factor 1 antibody Positive regulatory domain I-binding factor 1 antibody PR Domain Containing 1 antibody PR domain containing 1 with ZNF domain antibody PR domain containing 1 with ZNF domain isoform 2 antibody PR domain containing protein 1 antibody PR domain zinc finger protein 1 antibody PR domain-containing protein 1 antibody PRDI BF1 antibody PRDI binding factor 1 antibody PRDI-BF1 antibody PRDI-binding factor 1 antibody PRDM 1 antibody Prdm1 antibody PRDM1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 92 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
| SwissProt ID | O75626 |
|---|
Application
Fig1: Western blot analysis of PRDM1 on human PRDM1 recombinant protein using anti-PRDM1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PRDM1 on HEK293 (1) and PRDM1-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PRDM1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Raji cells with PRDM1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PRDM1 recombinant protein, PRDM1-hIgGFc transfected HEK293 cell lysate, Hela, Raji. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:1,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
