Application
Fig1: Western blot analysis of IRAKM on human IRAKM recombinant protein using anti- IRAKM antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of IRAKM on HEK293 (1) and IRAKM -hIgGFc transfected HEK293 (2) cell lysate using anti-IRAKM antibody at 1/1,000 dilution.Application
Fig3: ICC staining IRAKM (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining IRAKM (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with IRAKM antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | IRAK3 [G2-G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASRT5 antibody IL 1 receptor associated kinase M antibody IL-1 receptor-associated kinase M antibody IL1 receptor associated kinase M antibody Interleukin 1 receptor associated kinase 3 antibody Interleukin 1 receptor associated kinase M antibody Interleukin-1 receptor-associated kinase 3 antibody IRAK M antibody IRAK-3 antibody IRAK-M antibody Irak3 antibody IRAK3_HUMAN antibody |
| Product description | Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway. An IL-1-dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFkB activation. A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkB. IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFkB activation. IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells. IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ASRT5 antibody IL 1 receptor associated kinase M antibody IL-1 receptor-associated kinase M antibody IL1 receptor associated kinase M antibody Interleukin 1 receptor associated kinase 3 antibody Interleukin 1 receptor associated kinase M antibody Interleukin-1 receptor-associated kinase 3 antibody IRAK M antibody IRAK-3 antibody IRAK-M antibody Irak3 antibody IRAK3_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Cytoplasm, nucleus |
| SwissProt ID | Q9Y616 |
|---|
Application
Fig1: Western blot analysis of IRAKM on human IRAKM recombinant protein using anti- IRAKM antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of IRAKM on HEK293 (1) and IRAKM -hIgGFc transfected HEK293 (2) cell lysate using anti-IRAKM antibody at 1/1,000 dilution.
Application
Fig3: ICC staining IRAKM (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IRAKM (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with IRAKM antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human IRAKM recombinant protein, IRAKM -hIgGFc transfected HEK293 cell lysate, A549, Hela, HepG2, human cervical cancer tissue, human breast cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
