Application
Fig1: Western blot analysis of PLA2G12A on human PLA2G12A recombinant protein using anti-PLA2G12A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PLA2G12A on HEK293 (1) and PLA2G12A-hIgGFc transfected HEK293 (2) cell lysate using anti-PLA2G12A antibody at 1/1,000 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PLA2G12A antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Hela cells with PLA2G12A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PLA2G12A [C11-H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 3.1.1.4 antibody Group XII secreted phospholipase A2 antibody Group XIIA secreted phospholipase A2 antibody Group XIIA secretory phospholipase A2 antibody Group XIIA secretory phospholipase A2 precursor antibody GXII antibody GXII sPLA2 antibody PG12A_HUMAN antibody phosphatidylcholine 2 acylhydrolase 12A antibody Phosphatidylcholine 2 acylhydrolase GXII antibody Phosphatidylcholine 2-acylhydrolase 12A antibody Phospholipase A2 group XIIA antibody PLA2G12 antibody Pla2g12a antibody ROSSY antibody sPLA2 XII antibody sPLA2-XII antibody |
| Product description | Secreted phospholipases A2 (sPLA2s) form a large family of structurally related enzymes, which are widespread in nature. Snake venoms have been known for decades to contain a tremendous molecular diversity of sPLA2s, which can exert a myriad of toxic and pharmacological effects. Secreted phospholipase A2 enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as group XIIA sPLA2, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s. Group XIIA sPLA2, also known as GXII, ROSSY, PLA2G12 or PLA2G12A, is a 189 amino acid secreted protein that localizes to the cytoplasm. Belonging to the phospholipase A2 family, group XIIA sPLA2 is abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | EC 3.1.1.4 antibody Group XII secreted phospholipase A2 antibody Group XIIA secreted phospholipase A2 antibody Group XIIA secretory phospholipase A2 antibody Group XIIA secretory phospholipase A2 precursor antibody GXII antibody GXII sPLA2 antibody PG12A_HUMAN antibody phosphatidylcholine 2 acylhydrolase 12A antibody Phosphatidylcholine 2 acylhydrolase GXII antibody Phosphatidylcholine 2-acylhydrolase 12A antibody Phospholipase A2 group XIIA antibody PLA2G12 antibody Pla2g12a antibody ROSSY antibody sPLA2 XII antibody sPLA2-XII antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Secreted. Cytoplasm |
| SwissProt ID | Q9BZM1 |
|---|
Application
Fig1: Western blot analysis of PLA2G12A on human PLA2G12A recombinant protein using anti-PLA2G12A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PLA2G12A on HEK293 (1) and PLA2G12A-hIgGFc transfected HEK293 (2) cell lysate using anti-PLA2G12A antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PLA2G12A antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with PLA2G12A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PLA2G12A recombinant protein, PLA2G12A-hIgGFc transfected HEK293 cell lysate, human liver tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
