Application
Fig1: Western blot analysis of PPP1CB on human PPP1CB recombinant protein using anti-PPP1CB antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PPP1CB on HEK293 (1) and PPP1CB-hIgGFc transfected HEK293 (2) cell lysate using anti-PPP1CB antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PPP1CB on different cell lysate using anti-PPP1CB antibody at 1/1,000 dilution. Positive control： Line1: Jurkat Line2: A431 Line3: Hela Line4: HepG2 Line5: HEK293 Line6: MCF-7Application
Fig4: ICC staining PPP1CB (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-PPP1CB antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PPP1CB antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with PPP1CB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PPP1CB [A7-C7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC3672 antibody PP 1B antibody PP-1B antibody PP1B antibody PP1B_HUMAN antibody PP1beta antibody PPP1CB antibody PPP1CD antibody Protein phosphatase 1 beta antibody Protein phosphatase 1 catalytic subunit beta isoform antibody Protein phosphatase 1 delta antibody Protein phosphatase 1, catalytic subunit, beta isozyme antibody Protein phosphatase 1, catalytic subunit, delta isoform antibody Serine threonine protein phosphatase PP1 beta catalytic subunit antibody Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody |
| Product description | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP1 family is comprised of subfamily members PP1α, PP1β and PP1γ, which are MgATP-dependent enzymes. PP1 inactivity is maintained through its association with the inhibitory protein NIPP-1 (nuclear inhibitor of PP1). Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II results in the release of active PP1. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | MGC3672 antibody
PP 1B antibody
PP-1B antibody
PP1B antibody
PP1B_HUMAN antibody
PP1beta antibody
PPP1CB antibody
PPP1CD antibody
Protein phosphatase 1 beta antibody
Protein phosphatase 1 catalytic subunit beta isoform antibody
Protein phosphatase 1 delta antibody
Protein phosphatase 1 catalytic subunit beta isozyme antibody Protein phosphatase 1 catalytic subunit delta isoform antibody Serine threonine protein phosphatase PP1 beta catalytic subunit antibody Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cytoplasm. Nucleus |
| SwissProt ID | P62140 |
|---|
Application
Fig1: Western blot analysis of PPP1CB on human PPP1CB recombinant protein using anti-PPP1CB antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PPP1CB on HEK293 (1) and PPP1CB-hIgGFc transfected HEK293 (2) cell lysate using anti-PPP1CB antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PPP1CB on different cell lysate using anti-PPP1CB antibody at 1/1,000 dilution. Positive control： Line1: Jurkat Line2: A431 Line3: Hela Line4: HepG2 Line5: HEK293 Line6: MCF-7
Application
Fig4: ICC staining PPP1CB (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-PPP1CB antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PPP1CB antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with PPP1CB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PPP1CB recombinant protein, PPP1CB-hIgGFc transfected HEK293 cell lysate, Jurkat, A431, Hela, HepG2, HEK293, MCF-7, human cervical cancer tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
