Application
Fig1: Western blot analysis of PLCG1 on human PLCG1 recombinant protein using anti-PLCG1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PLCG1 on HEK293 (1) and PLCG1-hIgGFc transfected HEK293 (2) cell lysate using anti-PLCG1 antibody at 1/1,000 dilution.Application
f PLCG1 on different cell lysate using anti-PLCG1 antibody at 1/1,000 dilution.? Positive control： Line1: Hela Line2: A431 Line3: C6 Line4: NIH/3T3 Line5: COS7 Line6: HCT116Application
Fig4: ICC staining PLCG1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PLCG1 [D11-C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody Inositoltrisphosphohydrolase antibody Monophosphatidylinositol phosphodiesterase antibody NCKAP3 antibody Phosphatidylinositol phospholipase C antibody Phosphoinositidase C antibody Phosphoinositide phospholipase C antibody Phosphoinositide phospholipase C-gamma-1 antibody Phospholipase C 148 antibody Phospholipase C gamma 1 antibody Phospholipase C-gamma-1 antibody Phospholipase C-II antibody PLC gamma 1 antibody PLC II antibody PLC-148 antibody PLC-gamma-1 antibody PLC-II antibody PLC1 antibody PLC148 antibody Plcg1 antibody PLCG1_HUMAN antibody PLCgamma1 antibody |
| Product description | Phospholipase C-gamma 1 (PLC g1) is an isozyme of the phosphoinositide-specific PLC family, which occupies a central role in hormonal signal transduction pathways and is a substrate for the epidermal growth factor receptor tyrosine kinase. Following activation of TrkB, PLC g1 is phosphorylated on Tyrosine 783, Tyrosine 771 and Tyrosine 1253. Tyrosine 783 lies just downstream of the kinase domain in a relatively short sequence motif characteristic of the Trk family of protein-tyrosine kinase receptors. The sequence around Tyrosine 783 fits a consensus sequence for binding PLC g1. PLC g1 also forms a complex with TrkB consistent with the possibility that one of the TrkB autophosphorylation sites provides a binding site for the PLC g1 SH2 domains, as is the case for other receptor protein-tyrosine kinases. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanRatMonkey |
| Concentration | 2mg/ml |
| Alternative Names | 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-1 antibody Inositoltrisphosphohydrolase antibody Monophosphatidylinositol phosphodiesterase antibody NCKAP3 antibody Phosphatidylinositol phospholipase C antibody Phosphoinositidase C antibody Phosphoinositide phospholipase C antibody Phosphoinositide phospholipase C-gamma-1 antibody Phospholipase C 148 antibody Phospholipase C gamma 1 antibody Phospholipase C-gamma-1 antibody Phospholipase C-II antibody PLC gamma 1 antibody PLC II antibody PLC-148 antibody PLC-gamma-1 antibody PLC-II antibody PLC1 antibody PLC148 antibody Plcg1 antibody PLCG1_HUMAN antibody PLCgamma1 antibody |
|---|---|
| Molecular Weight(MW) | 148 kDa |
| Cellular Localization | Cell projection, lamellipodium, Cell projection, ruffle |
Application
Fig1: Western blot analysis of PLCG1 on human PLCG1 recombinant protein using anti-PLCG1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PLCG1 on HEK293 (1) and PLCG1-hIgGFc transfected HEK293 (2) cell lysate using anti-PLCG1 antibody at 1/1,000 dilution.
Application
f PLCG1 on different cell lysate using anti-PLCG1 antibody at 1/1,000 dilution.? Positive control： Line1: Hela Line2: A431 Line3: C6 Line4: NIH/3T3 Line5: COS7 Line6: HCT116
Application
Fig4: ICC staining PLCG1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PLCG1 recombinant protein,PLCG1-hIgGFc transfected HEK293 cell lysate, A431, Hela, C6, NIH/3T3, COS7, HCT116, human cervical cancer tissues, human bladder cancer tissues, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
