Application
Fig1: Western blot analysis of CDH11 on human CDH11 recombinant protein using anti-CDH11 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CDH11 on HEK293 (1) and CDH11-hIgGFc transfected HEK293 (2) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CDH11 on MCF-7 (1), Jurkat (2) and HEK293 (3) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.Application
Fig4: ICC staining CDH11 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CDH11 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with CDH11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CDH11 [A6-C12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAD11 antibody CAD11_HUMAN antibody Cadherin 11 antibody Cadherin 11 type 2 antibody Cadherin 11 type 2 OB cadherin (osteoblast) antibody Cadherin-11 antibody Cdh11 antibody CDHOB antibody OB antibody OB-cadherin antibody OBcadherin antibody OSF-4 antibody OSF4 antibody Osteoblast cadherin antibody |
| Product description | Cadherins are calcium dependent cell adhesion proteins, a family of Ca++-dependent adhesion molecules that influence cell-cell binding and are critical to the maintenance of tissue structure and morphogenesis. OB-cadherin (osteoblast-cadherin, cadherin-11, OSF-4) has two forms, OB-cadherin-1 and OB-cadherin-2. OB-cadherin-2 has a truncated cytoplasmic domain, missing amino acids 694-796. Both OB-cadherins are expressed in osteoblastic cell lines with low expression seen in lungs, testis and brain. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CAD11 antibody CAD11_HUMAN antibody Cadherin 11 antibody Cadherin 11 type 2 antibody Cadherin 11 type 2 OB cadherin (osteoblast) antibody Cadherin-11 antibody Cdh11 antibody CDHOB antibody OB antibody OB-cadherin antibody OBcadherin antibody OSF-4 antibody OSF4 antibody Osteoblast cadherin antibody |
|---|---|
| Molecular Weight(MW) | 88 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | P55287 |
|---|
Application
Fig1: Western blot analysis of CDH11 on human CDH11 recombinant protein using anti-CDH11 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CDH11 on HEK293 (1) and CDH11-hIgGFc transfected HEK293 (2) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CDH11 on MCF-7 (1), Jurkat (2) and HEK293 (3) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining CDH11 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CDH11 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with CDH11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CDH11 recombinant protein, CDH11-hIgGFc transfected HEK293 cell, MCF-7, Jurkat, HEK293, HL-7702, Hela, human bladder cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
