Application
Fig1: Western blot analysis of ALK on human ALK recombinant protein using anti-ALK antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ALK on HEK293 (1) and ALK -hIgGFc transfected HEK293 (2) cell lysate using anti-ALK antibody at 1/1,000 dilution.Application
Fig3: ICC staining ALK (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of Hela cells with ALK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ALK [A4-A11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alk antibody ALK tyrosine kinase receptor antibody ALK/EML4 fusion gene, included antibody ALK/NPM1 fusion gene, included antibody ALK_HUMAN antibody anaplastic lymphoma kinase (Ki-1) antibody Anaplastic lymphoma kinase antibody Anaplastic lymphoma kinase Ki1 antibody anaplastic lymphoma receptor tyrosine kinase antibody CD 246 antibody CD246 antibody CD246 antigen antibody EC 2.7.10.1 antibody Ki 1 antibody Ki1 antibody mutant anaplastic lymphoma kinase antibody NBLST 3 antibody NBLST3 antibody Tcrz antibody TFG/ALK antibody |
| Product description | LTK, ALK and Ros have been identified as receptor tyrosine kinases having sequence similarity to the insulin receptor subfamily of kinases. LTK (leukocyte tyrosine kinase) is expressed in murine B lymphocyte precursors and has also been found in forebrain neurons. ALK (anaplastic lymphoma kinase) is normally highly expressed, specifically in the nervous system. A truncated form containing the catalytic domain of ALK is expressed as the result of a translocation occuring in many non-Hodgkin's lymphomas. The c-Ros gene was originally identified in mutant form as an oncogene. Ros is normally expressed in a small number of epithelial cell types and may play a role in epithelial development. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Alk antibody
ALK tyrosine kinase receptor antibody
ALK/EML4 fusion gene included antibody ALK/NPM1 fusion gene included antibody ALK_HUMAN antibody anaplastic lymphoma kinase (Ki-1) antibody Anaplastic lymphoma kinase antibody Anaplastic lymphoma kinase Ki1 antibody anaplastic lymphoma receptor tyrosine kinase antibody CD 246 antibody CD246 antibody CD246 antigen antibody EC 2.7.10.1 antibody Ki 1 antibody Ki1 antibody mutant anaplastic lymphoma kinase antibody NBLST 3 antibody NBLST3 antibody Tcrz antibody TFG/ALK antibody |
|---|---|
| Molecular Weight(MW) | 176 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | Q9UM73 |
|---|
Application
Fig1: Western blot analysis of ALK on human ALK recombinant protein using anti-ALK antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ALK on HEK293 (1) and ALK -hIgGFc transfected HEK293 (2) cell lysate using anti-ALK antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ALK (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with ALK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ALK recombinant protein, ALK-hIgGFc transfected HEK293, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
