Application
Fig1: Western blot analysis of ATG13 on human ATG13 recombinant protein using anti-ATG13 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ATG13 on HEK293 (1) and ATG13-hIgGFc transfected HEK293 (2) cell lysate using anti-ATG13 antibody at 1/1,000 dilution.Application
Fig3: ICC staining ATG13 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with ATG13 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | ATG13 [C8-E4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG13 antibody ATG13 autophagy related 13 homolog (S. cerevisiae) antibody ATG13_HUMAN antibody Autophagy related 13 antibody Autophagy related protein 13 antibody Autophagy-related protein 13 antibody FLJ20698 antibody KIAA0652 antibody OTTHUMP00000233321 antibody OTTHUMP00000233322 antibody OTTHUMP00000233323 antibody OTTHUMP00000233324 antibody OTTHUMP00000233325 antibody OTTHUMP00000233326 antibody |
| Product description | Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1：50-1：100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ATG13 antibody ATG13 autophagy related 13 homolog (S. cerevisiae) antibody ATG13_HUMAN antibody Autophagy related 13 antibody Autophagy related protein 13 antibody Autophagy-related protein 13 antibody FLJ20698 antibody KIAA0652 antibody OTTHUMP00000233321 antibody OTTHUMP00000233322 antibody OTTHUMP00000233323 antibody OTTHUMP00000233324 antibody OTTHUMP00000233325 antibody OTTHUMP00000233326 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P13473 |
|---|
Application
Fig1: Western blot analysis of ATG13 on human ATG13 recombinant protein using anti-ATG13 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ATG13 on HEK293 (1) and ATG13-hIgGFc transfected HEK293 (2) cell lysate using anti-ATG13 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining ATG13 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with ATG13 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ATG13 recombinant protein, ATG13-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1：50-1：100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
