Application
Fig1: ICC staining SPHK2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Hela cells with SPHK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | SK2 [E1-C5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody ATP SULFURYLASE/ADENOSINE 5-PRIME PHOSPHOSULFATE KINASE 2 antibody ATP sulfurylase/APS kinase 2 antibody ATPSK2 antibody Bifunctional 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody Bifunctional 3' phosphoadenosine 5' phosphosulfate synthethase 2 antibody PAPS synthase 2 antibody PAPS synthetase 2 antibody PAPSS 2 antibody PHOSPHOADENOSINE-PHOSPHOSULFATE SYNTHETASE 2 antibody SK 2 antibody SK2 antibody Sulfurylase kinase 2 antibody |
| Product description | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICCIHCFC |
| ICC:1:200-1:1000 IHC:1:200-1:1000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody ATP SULFURYLASE/ADENOSINE 5-PRIME PHOSPHOSULFATE KINASE 2 antibody ATP sulfurylase/APS kinase 2 antibody ATPSK2 antibody Bifunctional 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody Bifunctional 3' phosphoadenosine 5' phosphosulfate synthethase 2 antibody PAPS synthase 2 antibody PAPS synthetase 2 antibody PAPSS 2 antibody PHOSPHOADENOSINE-PHOSPHOSULFATE SYNTHETASE 2 antibody SK 2 antibody SK2 antibody Sulfurylase kinase 2 antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa |
| Cellular Localization | Cytosol |
| SwissProt ID | Q9NRA0 |
|---|
Application
Fig1: ICC staining SPHK2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with SPHK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Hela, human bladder cancer tissue, human endometrial cancer tissue |
|---|---|
| Application Notes | ICC:1:200-1:1000 IHC:1:200-1:1000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
