Application
Fig1: Western blot analysis of ferritin on human ferritin recombinant protein using anti- ferritin antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of ferritin on HEK293 (1) and ferritin -hIgGFc transfected HEK293 (2) cell lysateusing using anti- ferritin antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of ferritin on K562 cells lysates using anti- ferritin antibody at 1/1,000 dilution.Application
Fig4: ICC staining ferritin (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- ferritin antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal tissue using anti- ferritin antibody. Counter stained with hematoxylin.| Product Name | FTL [E7-E1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ferritin L chain antibody Ferritin L subunit antibody Ferritin light chain antibody Ferritin light polypeptide antibody ferritin light polypeptide like 3 antibody FRIL_HUMAN antibody FTL antibody LFTD antibody NBIA 3 antibody NBIA3 antibody |
| Product description | Mammalian ferritins consist of 24 subunits made up of two types of polypeptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. The gene encoding human ferritin heavy chain maps to chromosome 11q13 and the human ferritin light chain gene maps to chromosome 19q13.3-q13.4. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Ferritin L chain antibody Ferritin L subunit antibody Ferritin light chain antibody Ferritin light polypeptide antibody ferritin light polypeptide like 3 antibody FRIL_HUMAN antibody FTL antibody LFTD antibody NBIA 3 antibody NBIA3 antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Autolysosome. azurophil granule lumen. |
Application
Fig1: Western blot analysis of ferritin on human ferritin recombinant protein using anti- ferritin antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ferritin on HEK293 (1) and ferritin -hIgGFc transfected HEK293 (2) cell lysateusing using anti- ferritin antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ferritin on K562 cells lysates using anti- ferritin antibody at 1/1,000 dilution.
Application
Fig4: ICC staining ferritin (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- ferritin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal tissue using anti- ferritin antibody. Counter stained with hematoxylin.| Positive Control | Recombinant protein, ferritin-hIgGFc transfected HEK293 cell .HEK293,K562,A431,human liver cancer, esophageal tissues. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
