Application
Fig1: Western blot analysis of PLCG2 on human PLCG2 recombinant protein using anti-PLCG2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PLCG2 on HEK293 (1) and PLCG2-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab3a antibody at 1/1,000 dilution.Application
Fig3: ICC staining PLCG2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with PLCG2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PLCG2 [E8-D9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody 1-phosphatidylinositol-4 antibody 5-bisphosphate phosphodiesterase gamma-2 antibody EC 3.1.4.11 antibody Phosphoinositide phospholipase C antibody Phosphoinositide phospholipase C-gamma-2 antibody Phospholipase C gamma 2 antibody Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody Phospholipase C-gamma-2 antibody Phospholipase C-IV antibody PLC 2 antibody PLC gamma 2 antibody PLC IV antibody PLC-gamma-2 antibody PLC-IV antibody Plcg2 antibody PLCG2_HUMAN antibody |
| Product description | Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC γ2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR γ chain), which bind to its C-terminal SH2 domain. PLC γ1 associates with Syk in B cells, but PLC γ2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC γ2. In addition, Btk can induce PLC γ2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
1-phosphatidylinositol-4 antibody
5-bisphosphate phosphodiesterase gamma-2 antibody
EC 3.1.4.11 antibody
Phosphoinositide phospholipase C antibody
Phosphoinositide phospholipase C-gamma-2 antibody
Phospholipase C gamma 2 antibody
Phospholipase C gamma 2 (phosphatidylinositol specific) antibody Phospholipase C-gamma-2 antibody Phospholipase C-IV antibody PLC 2 antibody PLC gamma 2 antibody PLC IV antibody PLC-gamma-2 antibody PLC-IV antibody Plcg2 antibody PLCG2_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 148 kDa |
| Cellular Localization | Cytosol, Autolysosome. |
| SwissProt ID | P16885 |
|---|
Application
Fig1: Western blot analysis of PLCG2 on human PLCG2 recombinant protein using anti-PLCG2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PLCG2 on HEK293 (1) and PLCG2-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab3a antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PLCG2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with PLCG2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PLCG2 recombinant protein, PLCG2-hIgGFc transfected HEK293 cell lysate, MCF-7, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
