Application
Fig1: Western blot analysis of CD168 on human CD168 recombinant protein using anti-CD168 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CD168 on HEK293 (1) and CD168-hIgGFc transfected HEK293 (2) cell lysate using anti-CD168 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of Hela cells with CD168 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CD168 [C9-F2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD168 antibody CD168 antigen antibody HMMR antibody HMMR_HUMAN antibody Hyaluronan mediated motility receptor antibody Hyaluronan-mediated motility receptor (RHAMM) antibody IHABP antibody Intracellular hyaluronic acid-binding protein antibody MGC119494 antibody MGC119495 antibody OTTHUMP00000196920 antibody Receptor for hyaluronan-mediated motility antibody RHAMM antibody |
| Product description | Hyaluronic acid (HA) is a nonsulfated glycosaminoglycan that regulates cell adhesion and migration. HA effects are mediated through two receptors, CD44 (also designated HCAM) and the receptor of hyaluronic acid mediated motility (RHAMM). RHAMM, also designated intracellular hyaluronic acid binding protein (IHABP) and CD168, is a matrix receptor, which is linked to the plasma membrane by a GPI anchor and regulates cell motility. RHAMM expression is upregulated in malignant lymphoid tissues and is subsequently implicated in tumor progression and metastasis formation, as well as signal transduction. Although still unclear, RHAMM is thought to exist as several isoforms ranging in size. A variant isoform, designated v4, is a protein that when over-expressed, is thought to be the cause of transformation and metastasis formation in fibroblasts |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD168 antibody CD168 antigen antibody HMMR antibody HMMR_HUMAN antibody Hyaluronan mediated motility receptor antibody Hyaluronan-mediated motility receptor (RHAMM) antibody IHABP antibody Intracellular hyaluronic acid-binding protein antibody MGC119494 antibody MGC119495 antibody OTTHUMP00000196920 antibody Receptor for hyaluronan-mediated motility antibody RHAMM antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa |
| Cellular Localization | Cell surface. Cytoplasm |
| SwissProt ID | O75330 |
|---|
Application
Fig1: Western blot analysis of CD168 on human CD168 recombinant protein using anti-CD168 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD168 on HEK293 (1) and CD168-hIgGFc transfected HEK293 (2) cell lysate using anti-CD168 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with CD168 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CD168 recombinant protein, CD168-hIgGFc transfected HEK293 cell lysate, Hela |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
