Application
Fig1: Western blot analysis of AFAP1L2 on human AFAP1L2 recombinant protein using anti-AFAP1L2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of AFAP1L2 on HEK293 (1) and AFAP1L2-hIgGFc transfected HEK293 (2) cell lysate using anti-AFAP1L2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of AFAP1L2 on A549 cell lysate using anti-AFAP1L2 antibody at 1/1,000 dilution.Application
Fig4:Flow cytometric analysis of Hela cells with AFAP1L2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | AFAP1L2 [D1-H6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Actin filament-associated protein 1-like 2 antibody AF1L2_HUMAN antibody AFAP1-like protein 2 antibody afap1l2 antibody CTB-1144G6.4 antibody KIAA1914 antibody XB130 antibody |
| Product description | Actin filament associated protein (AFAP-110) interacts directly with actin filaments through its C-terminal actin-binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. By linking signaling molecules to actin filaments, AFAP-110 provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. AFAP-1L2 (Actin filament-associated protein 1-like 2), also known as XB130, is a 818 amino acid cytoplasmic protein that contains two Pleckstrin homology (PH) domains, which are normally found in proteins involved in intracellular signaling. Like its relative AFAP110, AFAP-1L2 interacts with Src kinase and may play a role in Src-regulated transcription activation. AFAP-1L2 is expressed in thyroid and spleen and can also be detected at lower levels in lung, brain, pancreas and kidney. There are four isoforms of AFAP-1L2 that are produced as a result of alternative splicing events |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Actin filament-associated protein 1-like 2 antibody AF1L2_HUMAN antibody AFAP1-like protein 2 antibody afap1l2 antibody CTB-1144G6.4 antibody KIAA1914 antibody XB130 antibody |
|---|---|
| Molecular Weight(MW) | 91 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q8N4X5 |
|---|
Application
Fig1: Western blot analysis of AFAP1L2 on human AFAP1L2 recombinant protein using anti-AFAP1L2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of AFAP1L2 on HEK293 (1) and AFAP1L2-hIgGFc transfected HEK293 (2) cell lysate using anti-AFAP1L2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of AFAP1L2 on A549 cell lysate using anti-AFAP1L2 antibody at 1/1,000 dilution.
Application
Fig4:Flow cytometric analysis of Hela cells with AFAP1L2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human AFAP1L2 recombinant protein, AFAP1L2-hIgGFc transfected HEK293 cell lysate, A549, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
