Application
Fig1: Western blot analysis of RALB on human RALB recombinant protein using anti-RALB antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RALB on HEK293 (1) and RALB-hIgGFc transfected HEK293 (2) cell lysate using anti-RALB antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of RALB on different cell lysate using anti-RALB antibody at 1/1,000 dilution. Positive control： Lane1: HepG2 Lane2: COS7 Lane3:NIH/3T3 Lane4: A549 Lane5: U251 Lane6:HT-29 Lane7: HEK293 Lane8: LOVOApplication
Fig4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-RALB antibody. Counter stained with hematoxylin.Application
Fig5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using anti-RALB antibody. Counter stained with hematoxylin.Application
Fig6:Flow cytometric analysis of Hela cells with RALB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RALB [B4-F8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5730472O18Rik antibody dRalb antibody GTP binding protein antibody Ralb antibody RALB_HUMAN antibody RAS like protein B antibody Ras related GTP binding protein B antibody Ras-related protein Ral-B antibody v ral simian leukemia viral oncogene homolog B (ras related GTP binding protein) antibody v ral simian leukemia viral oncogene homolog B (ras related) antibody V ral simian leukemia viral oncogene homolog B antibody |
| Product description | Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 2mg/ml |
| Alternative Names | 5730472O18Rik antibody dRalb antibody GTP binding protein antibody Ralb antibody RALB_HUMAN antibody RAS like protein B antibody Ras related GTP binding protein B antibody Ras-related protein Ral-B antibody v ral simian leukemia viral oncogene homolog B (ras related GTP binding protein) antibody v ral simian leukemia viral oncogene homolog B (ras related) antibody V ral simian leukemia viral oncogene homolog B antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of RALB on human RALB recombinant protein using anti-RALB antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RALB on HEK293 (1) and RALB-hIgGFc transfected HEK293 (2) cell lysate using anti-RALB antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of RALB on different cell lysate using anti-RALB antibody at 1/1,000 dilution. Positive control： Lane1: HepG2 Lane2: COS7 Lane3:NIH/3T3 Lane4: A549 Lane5: U251 Lane6:HT-29 Lane7: HEK293 Lane8: LOVO
Application
Fig4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-RALB antibody. Counter stained with hematoxylin.
Application
Fig5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using anti-RALB antibody. Counter stained with hematoxylin.
Application
Fig6:Flow cytometric analysis of Hela cells with RALB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RALB recombinant protein, RALB-hIgGFc transfected HEK293, HepG2, COS7, NIH/3T3, A549, U251, HT-29, HEK293, LOVO, Hela, cervical cancer tissue, human bladder cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
