Application
Fig1: Western blot analysis of HLA-B on human HLA-B recombinant protein using anti-HLA-B antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HLA-B on HEK293 (1) and HLA-B-hIgGFc transfected HEK293 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of HLA-B on Ramos?(1) and A431 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.Application
Fig4:ICC staining HLA-B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of MCF-7 cells with HLA-B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | HLA-B [B10-G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AS antibody HLA B protein antibody HLA class I histocompatibility antigen, B alpha chain antibody HLA-B antibody HLA-B histocompatibility type antibody HLAB antibody leukocyte antigen class I-B antibody lymphocyte antigen antibody major histocompatibility complex, class I, B antibody MHC class I antigen GN00104 antibody MHC class I antigen HLA-B heavy chain antibody MHC class I antigen SHCHA antibody MHC Class I HLA heavy chain antibody MHC HLA-B cell surface glycoprotein antibody MHC HLA-B transmembrane glycoprotein antibody SPDA1 antibody |
| Product description | Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B and HLA-C encode membrane anchored heavy chains which heterodimerize with a light chain (β-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B and HLA-C alleles. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AS antibody
HLA B protein antibody
HLA class I histocompatibility antigen B alpha chain antibody HLA-B antibody HLA-B histocompatibility type antibody HLAB antibody leukocyte antigen class I-B antibody lymphocyte antigen antibody major histocompatibility complex class I B antibody MHC class I antigen GN00104 antibody MHC class I antigen HLA-B heavy chain antibody MHC class I antigen SHCHA antibody MHC Class I HLA heavy chain antibody MHC HLA-B cell surface glycoprotein antibody MHC HLA-B transmembrane glycoprotein antibody SPDA1 antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Membrane |
| SwissProt ID | P01889 |
|---|
Application
Fig1: Western blot analysis of HLA-B on human HLA-B recombinant protein using anti-HLA-B antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HLA-B on HEK293 (1) and HLA-B-hIgGFc transfected HEK293 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of HLA-B on Ramos?(1) and A431 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.
Application
Fig4:ICC staining HLA-B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of MCF-7 cells with HLA-B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human HLA-B recombinant protein, HLA-B-hIgGFc transfected HEK293, Ramos, A431, Hela, MCF-7, human ovarian cancer, human bladder cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
