Application
Fig1: Western blot analysis of SOD2 on human SOD2 recombinant protein using anti-SOD2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of SOD2 on HEK293 (1) and SOD2-hIgGFc transfected HEK293 (2) cell lysate using anti-SOD2 antibody at 1/1,000 dilution.Application
Fig3: ICC staining SOD2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-SOD2 antibody. Counter stained with hematoxylin.Application
Fig5:Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-SOD2 antibody. Counter stained with hematoxylin.Application
Fig6:Flow cytometric analysis of Hela cells with SOD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | SOD2 [D2-H3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Indophenoloxidase B antibody IPO B antibody IPOB antibody Manganese containing superoxide dismutase antibody Manganese SOD antibody Manganese superoxide dismutase antibody Mangano superoxide dismutase antibody Mn SOD antibody Mn superoxide dismutase antibody MNSOD antibody MVCD6 antibody SOD 2 antibody SOD2 antibody SODM_HUMAN antibody Superoxide dismutase [Mn] mitochondrial antibody Superoxide dismutase [Mn], mitochondrial antibody Superoxide dismutase 2 mitochondrial antibody |
| Product description | The superoxide dismutase family is composed of three metalloenzymes (SOD-1, SOD-2 and SOD-3) that catalyze the oxido-reduction of reactive oxygen species (ROS) such as superoxide anion. The SOD-2 precursor is a 222 amino acid protein that is encoded by nuclear chromatin, synthesized in the cytosol and imported posttranslationally into the mitochondrial matrix. Unlike SOD-1, which is a homodimeric cytosolic Cu-Zn enzyme, SOD-2 is a homotetrameric manganese enzyme (also known as MnSOD) that functions in the mitochondrion. ROS are implicated in a wide range of degenerative processes, including Alzheimer’s disease, Parkinson’s disease and ischemic heart disease. Homozygous mutant mice, which lack SOD-2, exhibit dilated cardiomyopathy, accumulation of lipid in liver and skeletal muscle, metabolic acidosis, oxidative DNA damage and respiratory chain deficiencies in heart and skeletal muscle. Polymorphisms in the SOD-2 gene have also been implicated in nonfamilial, idiopathic, dilated cardiomyopathy in humans. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Indophenoloxidase B antibody
IPO B antibody
IPOB antibody
Manganese containing superoxide dismutase antibody
Manganese SOD antibody
Manganese superoxide dismutase antibody
Mangano superoxide dismutase antibody
Mn SOD antibody
Mn superoxide dismutase antibody
MNSOD antibody
MVCD6 antibody
SOD 2 antibody
SOD2 antibody
SODM_HUMAN antibody
Superoxide dismutase [Mn] mitochondrial antibody
Superoxide dismutase [Mn] mitochondrial antibody Superoxide dismutase 2 mitochondrial antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Mitochondrion matrix |
| SwissProt ID | P04179 |
|---|
Application
Fig1: Western blot analysis of SOD2 on human SOD2 recombinant protein using anti-SOD2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SOD2 on HEK293 (1) and SOD2-hIgGFc transfected HEK293 (2) cell lysate using anti-SOD2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining SOD2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-SOD2 antibody. Counter stained with hematoxylin.
Application
Fig5:Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-SOD2 antibody. Counter stained with hematoxylin.
Application
Fig6:Flow cytometric analysis of Hela cells with SOD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SOD2 recombinant protein, SOD2-hIgGFc transfected HEK293 cell lysate, Hela, human ovarian cancer, human rectum cancer |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
