Application
Fig1: Western blot analysis of DDX1 on human DDX1 recombinant protein using anti-DDX1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of DDX1 on HEK293 (1) and DDX1-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of DDX1 on different cell lysate using anti-DDX1 antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: MCF-7 Lane3: A431 Lane4: PC-3 Lane5: JurkatApplication
Fig4:ICC staining DDX1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DDX1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-DDX1 antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of Hela cells with DDX1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DDX1 [B2-E5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP dependent helicase DDX1 antibody ATP-dependent RNA helicase DDX1 antibody DBP RB antibody DBP-RB antibody DDX1 antibody DDX1_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody DEAD box 1 antibody DEAD box polypeptide 1 antibody DEAD box protein 1 antibody DEAD box protein retinoblastoma antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody |
| Product description | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX1 mRNA has a widespread distribution in human fetal tissue, but is not uniformly expressed in all tissues. Chicken DDX1, which shares 93% identity with human DDX1, shows highest levels of expression during the early stages of development. Tissue maturation typically correlates with a decrease in DDX1 expression, although DDX1 levels remain elevated in late embryonic retina and brain. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ATP dependent helicase DDX1 antibody ATP-dependent RNA helicase DDX1 antibody DBP RB antibody DBP-RB antibody DDX1 antibody DDX1_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody DEAD box 1 antibody DEAD box polypeptide 1 antibody DEAD box protein 1 antibody DEAD box protein retinoblastoma antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasmic granule. |
| SwissProt ID | Q92499 |
|---|
Application
Fig1: Western blot analysis of DDX1 on human DDX1 recombinant protein using anti-DDX1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DDX1 on HEK293 (1) and DDX1-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of DDX1 on different cell lysate using anti-DDX1 antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: MCF-7 Lane3: A431 Lane4: PC-3 Lane5: Jurkat
Application
Fig4:ICC staining DDX1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DDX1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-DDX1 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Hela cells with DDX1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DDX1 recombinant protein, DDX1-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7, A431, PC-3, Jurkat, human esophageal cancer, human cervical cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
