Application
Fig1: Western blot analysis of TRIM25 on human TRIM25 recombinant protein using anti-TRIM25 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of TRIM25 on HEK293 (1) and TRIM25-hIgGFc transfected HEK293 (2) cell lysate using anti-TRIM25 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of TRIM25 on different cell lysate using anti-TRIM25 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: MCF-7 Lane 3: K562 Lane 4: A549 Lane 5: MOLT4Application
Fig4: ICC staining TRIM25 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-TRIM25 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-TRIM25 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with TRIM25 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | TRIM25 [B12-B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin/ISG15 ligase TRIM25 antibody EFP antibody Estrogen responsive finger protein antibody Estrogen-responsive finger protein antibody RING finger protein 147 antibody RNF 147 antibody RNF147 antibody TRI25 antibody TRI25_HUMAN antibody TRIM 25 antibody Trim25 antibody Tripartite motif containing 25 antibody Tripartite motif containing protein 25 antibody Tripartite motif protein TRIM25 antibody Tripartite motif-containing protein 25 antibody Tripartite motifcontaining25 antibody Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody Z147 antibody Zinc finger protein 147 (estrogen responsive finger protein) antibody Zinc finger protein 147 antibody Zinc finger protein-147 antibody ZNF 147 antibody ZNF147 antibody |
| Product description | TRIM25 also named EFP. EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen. It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger. EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family. Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues; however, few genes have been shown to be directly regulated by estrogen. It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression. The EFP gene product may then activate transcription of secondary estrogen responsive genes. Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | E3 ubiquitin/ISG15 ligase TRIM25 antibody EFP antibody Estrogen responsive finger protein antibody Estrogen-responsive finger protein antibody RING finger protein 147 antibody RNF 147 antibody RNF147 antibody TRI25 antibody TRI25_HUMAN antibody TRIM 25 antibody Trim25 antibody Tripartite motif containing 25 antibody Tripartite motif containing protein 25 antibody Tripartite motif protein TRIM25 antibody Tripartite motif-containing protein 25 antibody Tripartite motifcontaining25 antibody Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody Z147 antibody Zinc finger protein 147 (estrogen responsive finger protein) antibody Zinc finger protein 147 antibody Zinc finger protein-147 antibody ZNF 147 antibody ZNF147 antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q14258 |
|---|
Application
Fig1: Western blot analysis of TRIM25 on human TRIM25 recombinant protein using anti-TRIM25 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of TRIM25 on HEK293 (1) and TRIM25-hIgGFc transfected HEK293 (2) cell lysate using anti-TRIM25 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of TRIM25 on different cell lysate using anti-TRIM25 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: MCF-7 Lane 3: K562 Lane 4: A549 Lane 5: MOLT4
Application
Fig4: ICC staining TRIM25 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-TRIM25 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-TRIM25 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with TRIM25 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human TRIM25 recombinant protein, TRIM25-hIgGFc transfected HEK293 cell lysate, MCF-7, K562, A549, MOLT4, Hela, human bladder cancer,human ?rectum cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
