Application
Fig1: Western blot analysis of KDM3A on human KDM3A recombinant protein using anti-KDM3A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of KDM3A on HEK293 (1) and KDM3A-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM3A antibody at 1/1,000 dilution.Application
Fig3: ICC staining KDM3A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of Hela cells with KDM3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | KDM3A [C2-G10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686A24246 antibody DKFZp686P07111 antibody JHDM2A antibody JHMD2A antibody JmjC domain-containing histone demethylation protein 2A antibody JMJD1 antibody JMJD1A antibody Jumonji C domain containing histone demethylase 2A antibody Jumonji domain containing 1 antibody Jumonji domain containing 1A antibody Jumonji domain containing protein 1A antibody Jumonji domain-containing protein 1A antibody Kdm3a antibody KDM3A_HUMAN antibody KIAA0742 antibody Lysine (K) specific demethylase 3A antibody Lysine-specific demethylase 3A antibody Testis specific protein A antibody TSGA antibody |
| Product description | JMJD1A (Jumonji domain containing 1A), also known as TSGA (testis-specific protein A), JMJD1, KDM3A, JHDM2A (JMJC domain-containing histone demethylation protein 2A) or JHMD2A, is a member of the JHDM2 histone demethylase family of proteins that is predominantly expressed in testis. Containing one JMJC domain and a C-terminal C2HC4 zinc finger, JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and a-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | DKFZp686A24246 antibody DKFZp686P07111 antibody JHDM2A antibody JHMD2A antibody JmjC domain-containing histone demethylation protein 2A antibody JMJD1 antibody JMJD1A antibody Jumonji C domain containing histone demethylase 2A antibody Jumonji domain containing 1 antibody Jumonji domain containing 1A antibody Jumonji domain containing protein 1A antibody Jumonji domain-containing protein 1A antibody Kdm3a antibody KDM3A_HUMAN antibody KIAA0742 antibody Lysine (K) specific demethylase 3A antibody Lysine-specific demethylase 3A antibody Testis specific protein A antibody TSGA antibody |
|---|---|
| Molecular Weight(MW) | 147 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q9Y4C1 |
|---|
Application
Fig1: Western blot analysis of KDM3A on human KDM3A recombinant protein using anti-KDM3A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of KDM3A on HEK293 (1) and KDM3A-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM3A antibody at 1/1,000 dilution.
Application
Fig3: ICC staining KDM3A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with KDM3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human KDM3A recombinant protein, KDM3A-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
