Application
Fig1: Western blot analysis of MIB1 on ?human MIB1 recombinant protein using anti-MIB1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of MIB1 on HEK293 (1) and MIB1-hIgGFc transfected HEK293 (2) cell lysate using anti-MIB1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of MIB1 on Hela (1) and COS7 (2) cell lysate using anti-MIB1 antibody at 1/1,000 dilution.Application
Fig4:ICC staining MIB1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5:Flow cytometric analysis of Hela cells with MIB1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | MIB1 [C9-A9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAPK-interacting protein 1 antibody Dip 1 antibody DIP-1 antibody Dip1 antibody E3 ubiquitin protein ligase MIB 1 antibody E3 ubiquitin protein ligase MIB1 antibody E3 ubiquitin-protein ligase mib1 antibody KIAA1323 antibody LVNC7 antibody MIB antibody mib1 antibody MIB1_HUMAN antibody Mind bomb homolog 1 antibody Mindbomb E3 ubiquitin protein ligase 1 antibody Ubiquitin ligase mind bomb antibody Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody ZZANK2 antibody ZZZ6 antibody |
| Product description | The LIN-12/notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. MIB1 (E3 ubiquitin-protein ligase MIB1), also known as Mind bomb homolog 1 and DAPK-interacting protein 1, is a 1006 amino acid E3 ubiquitin ligase that activates the Notch ligand, Delta. MIB1 ubiquinates Delta by binding to its intracellular domain, leading to the endocytosis and eventual degradation of the Delta receptor, which, paradoxically, results in the up-regulation of receptor activity and enhances Notch signaling. MIB1 also interacts with DAPK, a protein that plays an important role in the regulation of apoptosis. Ubiquination of DAPK leads to inhibition of caspase-dependent apoptosis, therefore it is likely that overexpression of MIB1 can lead to tumor growth. Although it seems to be widely expressed at low levels, MIB1 is expressed at highest concentrations in the CNS and ovary. Both DAPK and MIB1 are overexpressed in epileptic brain tissue, suggesting that they probably cooperate as regulators of neuronal death in epilepsy. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 2mg/ml |
| Alternative Names | DAPK-interacting protein 1 antibody Dip 1 antibody DIP-1 antibody Dip1 antibody E3 ubiquitin protein ligase MIB 1 antibody E3 ubiquitin protein ligase MIB1 antibody E3 ubiquitin-protein ligase mib1 antibody KIAA1323 antibody LVNC7 antibody MIB antibody mib1 antibody MIB1_HUMAN antibody Mind bomb homolog 1 antibody Mindbomb E3 ubiquitin protein ligase 1 antibody Ubiquitin ligase mind bomb antibody Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody ZZANK2 antibody ZZZ6 antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Cytoplasm. Cytoskeleton. Cell membrane |
| SwissProt ID | Q86YT6 |
|---|
Application
Fig1: Western blot analysis of MIB1 on ?human MIB1 recombinant protein using anti-MIB1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of MIB1 on HEK293 (1) and MIB1-hIgGFc transfected HEK293 (2) cell lysate using anti-MIB1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of MIB1 on Hela (1) and COS7 (2) cell lysate using anti-MIB1 antibody at 1/1,000 dilution.
Application
Fig4:ICC staining MIB1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5:Flow cytometric analysis of Hela cells with MIB1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human MIB1 recombinant protein, MIB1-hIgGFc transfected HEK293 cell lysate, Hela, Cos7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
