Application
Fig1: Western blot analysis of PTPN14 on human PTPN14 ecombinant protein using anti-PTPN14 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PTPN14 on HEK293 (1) and PTPN14-hIgGFc transfected HEK293 (2) cell lysate using anti-PTPN14 antibody at 1/1,000 dilution.Application
Fig3:ICC staining PTPN14 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with PTPN14 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PTPN14 [H7-B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytoskeletal associated protein tyrosine phosphatase antibody MGC126803 antibody PEZ antibody Phosphatase with ezrin domain antibody Protein tyrosine phosphatase non receptor type 14 antibody Protein tyrosine phosphatase pez antibody Protein-tyrosine phosphatase pez antibody PTN14_HUMAN antibody PTP 36 antibody PTP36 antibody PTPD 2 antibody PTPN 14 antibody PTPN14 antibody Tyrosine protein phosphatase non receptor type 14 antibody Tyrosine-protein phosphatase non-receptor type 14 antibody |
| Product description | Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Cytoskeletal associated protein tyrosine phosphatase antibody MGC126803 antibody PEZ antibody Phosphatase with ezrin domain antibody Protein tyrosine phosphatase non receptor type 14 antibody Protein tyrosine phosphatase pez antibody Protein-tyrosine phosphatase pez antibody PTN14_HUMAN antibody PTP 36 antibody PTP36 antibody PTPD 2 antibody PTPN 14 antibody PTPN14 antibody Tyrosine protein phosphatase non receptor type 14 antibody Tyrosine-protein phosphatase non-receptor type 14 antibody |
|---|---|
| Molecular Weight(MW) | 135 kDa |
| Cellular Localization | Cytoplasm. Cytoskeleton. |
| SwissProt ID | Q15678 |
|---|
Application
Fig1: Western blot analysis of PTPN14 on human PTPN14 ecombinant protein using anti-PTPN14 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PTPN14 on HEK293 (1) and PTPN14-hIgGFc transfected HEK293 (2) cell lysate using anti-PTPN14 antibody at 1/1,000 dilution.
Application
Fig3:ICC staining PTPN14 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with PTPN14 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PTPN14 ecombinant protein, PTPN14-hIgGFc transfected HEK293 cell lysate, Hela, human breast cancer tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
