Application
Fig1: Western blot analysis of PLD2 on ?human PLD2 recombinant protein using anti-PLD2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PLD2 on HEK293 (1) and PLD2-hIgGFc transfected HEK293 (2) cell lysate using anti-PLD2 antibody at 1/1,000 dilution.Application
Fig3:ICC staining PLD2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:ICC staining PLD2 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human renal tissues using anti-PLD2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using anti-PLD2 antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of Hela cells with PLD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PLD2 [D9-E4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Choline phosphatase 2 antibody EC 3.1.4.4 antibody hPLD 2 antibody hPLD2 antibody mPLD2 antibody Phosphatidylcholine hydrolyzing phospholipase D2 antibody Phosphatidylcholine-hydrolyzing phospholipase D2 antibody Phospholipase D2 antibody PhospholipaseD2 antibody PLD 2 antibody PLD1C antibody Pld2 antibody PLD2_HUMAN antibody Pldc antibody rPLD2 antibody |
| Product description | The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Choline phosphatase 2 antibody EC 3.1.4.4 antibody hPLD 2 antibody hPLD2 antibody mPLD2 antibody Phosphatidylcholine hydrolyzing phospholipase D2 antibody Phosphatidylcholine-hydrolyzing phospholipase D2 antibody Phospholipase D2 antibody PhospholipaseD2 antibody PLD 2 antibody PLD1C antibody Pld2 antibody PLD2_HUMAN antibody Pldc antibody rPLD2 antibody |
|---|---|
| Molecular Weight(MW) | 106 kDa |
| Cellular Localization | Membrane. Peripheral membrane protein. |
| SwissProt ID | O14939 |
|---|
Application
Fig1: Western blot analysis of PLD2 on ?human PLD2 recombinant protein using anti-PLD2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PLD2 on HEK293 (1) and PLD2-hIgGFc transfected HEK293 (2) cell lysate using anti-PLD2 antibody at 1/1,000 dilution.
Application
Fig3:ICC staining PLD2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:ICC staining PLD2 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human renal tissues using anti-PLD2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using anti-PLD2 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Hela cells with PLD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PLD2 recombinant protein, PLD2-hIgGFc transfected HEK293 cell lysate, MCF-7, SK-OV-3, Hela, human esophageal cancer, human renal |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
