Application
Fig1: Western blot analysis of BUB1 on human BUB1 recombinant protein using anti-BUB1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of BUB1 on HEK293 (1) and BUB1-hIgGFc transfected HEK293 (2) cell lysate using anti-BUB1 antibody at 1/1,000 dilution.Application
Fig3:Flow cytometric analysis of Hela cells with BUB1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | BUB1 [C7-A11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bub1 antibody BUB1 budding uninhibited by benzimidazoles 1 homolog antibody BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody BUB1 mitotic checkpoint serine/threonine kinase antibody BUB1, S. cerevisiae, homolog of antibody BUB1_HUMAN antibody BUB1A antibody BUB1L antibody Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody Budding uninhibited by benzimidazoles 1 homolog antibody Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody hBUB1 antibody Homolog of mitotic checkpoint gene BUB1 antibody Mitotic checkpoint gene BUB1 antibody Mitotic checkpoint serine/threonine protein kinase BUB1 antibody Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody Mitotic spindle checkpoint kinase antibody Putative serine/threonine protein kinase antibody |
| Product description | Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Bub1 antibody
BUB1 budding uninhibited by benzimidazoles 1 homolog antibody
BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody
BUB1 mitotic checkpoint serine/threonine kinase antibody
BUB1 S. cerevisiae homolog of antibody BUB1_HUMAN antibody BUB1A antibody BUB1L antibody Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody Budding uninhibited by benzimidazoles 1 homolog antibody Budding uninhibited by benzimidazoles 1 S. cerevisiae homolog of antibody hBUB1 antibody Homolog of mitotic checkpoint gene BUB1 antibody Mitotic checkpoint gene BUB1 antibody Mitotic checkpoint serine/threonine protein kinase BUB1 antibody Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody Mitotic spindle checkpoint kinase antibody Putative serine/threonine protein kinase antibody |
|---|---|
| Molecular Weight(MW) | 122 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O43683 |
|---|
Application
Fig1: Western blot analysis of BUB1 on human BUB1 recombinant protein using anti-BUB1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of BUB1 on HEK293 (1) and BUB1-hIgGFc transfected HEK293 (2) cell lysate using anti-BUB1 antibody at 1/1,000 dilution.
Application
Fig3:Flow cytometric analysis of Hela cells with BUB1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human BUB1 recombinant protein, BUB1-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
