Application
Fig1: Western blot analysis of Rab6b on human Rab6b recombinant protein using anti-Rab6b antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Rab6b on HEK293 (1) and Rab6b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab6b antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of Rab6b on different cell lysate using anti-Rab6b antibody at 1/1,000 dilution. Positive control： Lane1: C6 Lane2: HT-29 Lane3: PC-12Application
Fig4:ICC staining Rab6b (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining Rab6b (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.Application
Fig8:Flow cytometric analysis of Hela cells with Rab6b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | Rab6b [E4-D12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 9630015C03 antibody AI844641 antibody C330006L04Rik antibody D9Bwg0185e antibody OTTHUMP00000216372 antibody RAB6B antibody RAB6B member RAS oncogene family antibody RAB6B_HUMAN antibody Ras related protein Rab 6B antibody Ras-related protein Rab-6B antibody Small GTP binding protein antibody Small GTPase RAB6B antibody |
| Product description | Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. RAB6B (RAB6B, Member RAS Oncogene Family) is a Protein Coding gene. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is RAB41 |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | 9630015C03 antibody AI844641 antibody C330006L04Rik antibody D9Bwg0185e antibody OTTHUMP00000216372 antibody RAB6B antibody RAB6B member RAS oncogene family antibody RAB6B_HUMAN antibody Ras related protein Rab 6B antibody Ras-related protein Rab-6B antibody Small GTP binding protein antibody Small GTPase RAB6B antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Golgi apparatus membrane. Cytoplasmic vesicle |
| SwissProt ID | Q9NRW1 |
|---|
Application
Fig1: Western blot analysis of Rab6b on human Rab6b recombinant protein using anti-Rab6b antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Rab6b on HEK293 (1) and Rab6b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab6b antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of Rab6b on different cell lysate using anti-Rab6b antibody at 1/1,000 dilution. Positive control： Lane1: C6 Lane2: HT-29 Lane3: PC-12
Application
Fig4:ICC staining Rab6b (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining Rab6b (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.
Application
Fig8:Flow cytometric analysis of Hela cells with Rab6b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Rab6b recombinant protein, Rab6b-hIgGFc transfected HEK293 cell lysate, C6, HT-29, PC-12, Hela, HepG2, human ovarian cancer tissue, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
