Application
Fig1: Western blot analysis of PSMC3 on human PSMC3 recombinant protein using anti-PSMC3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PSMC3 on HEK293 (1) and PSMC3-hIgGFc transfected HEK293 (2) cell lysate using anti-PSMC3 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of PSMC3 on different cell lysate using anti-PSMC3 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: PC-3 Lane3: T47D Lane4: SW620 Lane5: COS7 Lane6: C6 Lane7: HELA Lane8: A431Application
Fig4:ICC staining PSMC3 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining PSMC3 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-PSMC3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PSMC3 antibody. Counter stained with hematoxylin.Application
Fig8:Flow cytometric analysis of Hela cells with PSMC3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PSMC3 [C9-G10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 26S protease regulatory subunit 6A antibody 26S proteasome AAA-ATPase subunit RPT5 antibody Human immunodeficiency virus tat transactivator binding protein 1 antibody MGC8487 antibody Proteasome (prosome macropain) 26S subunit ATPase 3 antibody Proteasome 26S ATPase subunit 3 antibody Proteasome 26S subunit ATPase 3 antibody Proteasome subunit P50 antibody PRS6A_HUMAN antibody PSMC 3 antibody PSMC3 antibody Tat binding protein 1 antibody TAT-binding protein 1 antibody TBP-1 antibody TBP1 antibody |
| Product description | In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S Proteasome. The 26S Proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains. It is made up of two multisubunit complexes: the 20S Proteasome chamber, which serves as the proteolytic core of the complex, and two 19S regulatory particles which recognize and unfold ubiquitinated proteins. PSMC3 (Proteasome 26S subunit ATPase 3), also known as TBP1 (Tat-binding protein 1), is a 439 amino acid member of the AAA ATPase family. Localized to both the nucleus and the cytoplasm, PSMC3 functions as a subunit of the 19S regulatory complex and is involved in regulating the substrate specificity of the 26S Proteasome. Additionally, PSMC3 interacts with the HIV protein HIV-1 Tat and, via this interaction, mediates the association of the viral protein with transcription complexes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMonkeyRat |
| Concentration | 2mg/ml |
| Alternative Names | 26S protease regulatory subunit 6A antibody 26S proteasome AAA-ATPase subunit RPT5 antibody Human immunodeficiency virus tat transactivator binding protein 1 antibody MGC8487 antibody Proteasome (prosome macropain) 26S subunit ATPase 3 antibody Proteasome 26S ATPase subunit 3 antibody Proteasome 26S subunit ATPase 3 antibody Proteasome subunit P50 antibody PRS6A_HUMAN antibody PSMC 3 antibody PSMC3 antibody Tat binding protein 1 antibody TAT-binding protein 1 antibody TBP-1 antibody TBP1 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Cytoplasm. Nucleus |
Application
Fig1: Western blot analysis of PSMC3 on human PSMC3 recombinant protein using anti-PSMC3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PSMC3 on HEK293 (1) and PSMC3-hIgGFc transfected HEK293 (2) cell lysate using anti-PSMC3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PSMC3 on different cell lysate using anti-PSMC3 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: PC-3 Lane3: T47D Lane4: SW620 Lane5: COS7 Lane6: C6 Lane7: HELA Lane8: A431
Application
Fig4:ICC staining PSMC3 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining PSMC3 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-PSMC3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PSMC3 antibody. Counter stained with hematoxylin.
Application
Fig8:Flow cytometric analysis of Hela cells with PSMC3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PSMC3 recombinant protein, PSMC3-hIgGFc transfected HEK293 cell lysate, MCF-7, PC-3, T47D, SW620, COS7, C6, HELA, A431, SK-OV-3, human breast cancer tissue, human bladder cancer tissue |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
