Application
Fig1: Western blot analysis of BNIP3 on human BNIP3 recombinant protein using anti-BNIP3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of BNIP3 on HEK293 (1) and BNIP3-hIgGFc transfected HEK293 (2) cell lysate using anti-BNIP3 antibody at 1/1,000 dilution.Application
Fig3: ICC staining BNIP3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using anti-BNIP3 antibody. Counter stained with hematoxylin.Application
Fig5:Immunohistochemical analysis of paraffin-embedded human ?rectum cancer tissues using anti-BNIP3 antibody. Counter stained with hematoxylin.Application
Flow cytometric analysis of Hela cells with BNIP3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | BNIP3 [F7-A5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody BNIP 3 antibody BNIP3 antibody BNIP3_HUMAN antibody NIP 3 antibody NIP3 antibody |
| Product description | The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. BNIP-3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the anti-apoptosis activity of Bcl-xL. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody BNIP 3 antibody BNIP3 antibody BNIP3_HUMAN antibody NIP 3 antibody NIP3 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Mitochondrion. Mitochondrion membrane |
| SwissProt ID | Q12983 |
|---|
Application
Fig1: Western blot analysis of BNIP3 on human BNIP3 recombinant protein using anti-BNIP3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of BNIP3 on HEK293 (1) and BNIP3-hIgGFc transfected HEK293 (2) cell lysate using anti-BNIP3 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining BNIP3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using anti-BNIP3 antibody. Counter stained with hematoxylin.
Application
Fig5:Immunohistochemical analysis of paraffin-embedded human ?rectum cancer tissues using anti-BNIP3 antibody. Counter stained with hematoxylin.
Application
Flow cytometric analysis of Hela cells with BNIP3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human BNIP3 recombinant protein, BNIP3-hIgGFc transfected HEK293 cell lysate, Hela, human breast cancer tissue, human ?rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
