Application
Fig1: Western blot analysis of SYK on human SYK recombinant protein using anti-SYK antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of SYK on HEK293 (1) and SYK-hIgGFc transfected HEK293 (2) cell lysate using anti-SYK antibody at 1/1,000 dilution.Application
Fig3: ICC staining SYK (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Flow cytometric analysis of Hela cells with SYK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | SYK [E6-G11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 2.7.10.2 antibody kinase Syk antibody KSYK antibody KSYK_HUMAN antibody p72-Syk antibody p72syk antibody Spleen tyrosine kinase antibody Syk antibody Tyrosine protein kinase SYK antibody Tyrosine-protein kinase SYK antibody |
| Product description | Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains. One of several members of the protein kinase superfamily, Syk functions as a positive effector of B cell antigen receptor (CD79)-stimulated responses, coupling CD79 with the movement of one calcium ion through one of two phospho-regulated pathways. Specifically, calcium ions travel through either a phosphoinositide 3-kinase (PI 3-kinase)-dependent pathway when Syk is not phosphorylated, or through a phospholipase C (PLC) γ-dependent pathway when human Syk is phohsphorylated on Tyr 348 and Tyr 352. Via its ability to influence CD79 activity and to control the movement of calicum through the cell, Syk plays an important role in a variety of cellular responses, including differentiation, phagocytosis, proliferation and B cell development. Syk expression is upregulated in T cell lymphoma, suggesting a possible role for Syk in tumorigenesis. Two isoforms of Syk, designated short and long, exist due to alternative splicing events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | EC 2.7.10.2 antibody kinase Syk antibody KSYK antibody KSYK_HUMAN antibody p72-Syk antibody p72syk antibody Spleen tyrosine kinase antibody Syk antibody Tyrosine protein kinase SYK antibody Tyrosine-protein kinase SYK antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Cell membrane. Cytoplasm |
| SwissProt ID | P43405 |
|---|
Application
Fig1: Western blot analysis of SYK on human SYK recombinant protein using anti-SYK antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SYK on HEK293 (1) and SYK-hIgGFc transfected HEK293 (2) cell lysate using anti-SYK antibody at 1/1,000 dilution.
Application
Fig3: ICC staining SYK (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with SYK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SYK recombinant protein, SYK-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
