Application
Fig1: Western blot analysis of CDCP1 on human CDCP1 recombinant protein using anti-CDCP1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CDCP1 on HEK293 (1) and CDCP1-hIgGFc transfected HEK293 (2) cell lysate using anti-CDCP1 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of Hela cells with CDCP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CDCP1 [G3-H11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 9030022E12Rik antibody AA409659 antibody CD 318 antibody CD_antigen=CD318 antibody CD318 antibody CD318 antigen antibody CDCP 1 antibody Cdcp1 antibody CDCP1_HUMAN antibody CUB domain containing protein 1 antibody CUB domain-containing protein 1 antibody E030027H19Rik antibody FLJ13772 antibody FLJ22375 antibody FLJ22969 antibody Membrane glycoprotein gp140 antibody MGC31813 antibody OTTHUMP00000164120 antibody OTTHUMP00000209568 antibody RGD1305578 antibody SIMA 135 antibody SIMA135 antibody Subtractive immunization M plus HEp3 associated 135 kDa protein antibody Subtractive immunization M plus HEp3-associated 135 kDa protein antibody Transmembrane and associated with src kinases antibody TRASK antibody |
| Product description | CDCP1 (CUB-domain-containing protein 1) contains three extracellular CUB domains, a transmembrane domain, and two putative cytoplasmic tyrosine phosphorylation sites. Phosphorylation of the gp140 and p80 proteins is mediated by Src family kinases at various tyrosine residues, including Tyr 734. PTP family members mediate the desphosphorylation of CDCP1. The conversion of gp140 to p80 prolongs the phosphorylation state, which may affect signaling in epithelial wounds. CDCP1 acts as a marker for hematopoetic cells and also exhibits high expression in metastatic colon and breast tumors. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:200-1:500 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 9030022E12Rik antibody AA409659 antibody CD 318 antibody CD_antigen=CD318 antibody CD318 antibody CD318 antigen antibody CDCP 1 antibody Cdcp1 antibody CDCP1_HUMAN antibody CUB domain containing protein 1 antibody CUB domain-containing protein 1 antibody E030027H19Rik antibody FLJ13772 antibody FLJ22375 antibody FLJ22969 antibody Membrane glycoprotein gp140 antibody MGC31813 antibody OTTHUMP00000164120 antibody OTTHUMP00000209568 antibody RGD1305578 antibody SIMA 135 antibody SIMA135 antibody Subtractive immunization M plus HEp3 associated 135 kDa protein antibody Subtractive immunization M plus HEp3-associated 135 kDa protein antibody Transmembrane and associated with src kinases antibody TRASK antibody |
|---|---|
| Molecular Weight(MW) | 93 kDa |
| Cellular Localization | Secreted. Cell membrane |
| SwissProt ID | Q9H5V8 |
|---|
Application
Fig1: Western blot analysis of CDCP1 on human CDCP1 recombinant protein using anti-CDCP1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CDCP1 on HEK293 (1) and CDCP1-hIgGFc transfected HEK293 (2) cell lysate using anti-CDCP1 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with CDCP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CDCP1 recombinant protein, CDCP1-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:200-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
