Application
Fig1: Western blot analysis of TTF1 on ?human TTF1 recombinant protein using anti-TTF1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of TTF1 on HEK293 (1) and TTF1-hIgGFc transfected HEK293 (2) cell lysate using anti-TTF1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining TTF1 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:ICC staining TTF1 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-TTF1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-TTF1 antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of Hela cells with TTF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | TTF1 [D8-F4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AV026640 antibody BCH antibody Benign chorea antibody BHC antibody Homeobox protein NK 2 homolog A antibody Homeobox protein NK-2 homolog A antibody Homeobox protein Nkx 2.1 antibody Homeobox protein Nkx-2.1 antibody Homeobox protein Nkx2.1 antibody NK 2 antibody NK 2 homolog A antibody NK2 antibody NK2 homeobox 1 antibody NK2, drosophila, homolog of, A antibody NK2.1, mouse, homolog of antibody Nkx 2 1 antibody NKX 2.1 antibody NKX 2A antibody NKX2 1 antibody Nkx2-1 antibody NKX2.1 antibody NKX21_HUMAN antibody NKX2A antibody T EBP antibody T/EBP antibody TEBP antibody Thyroid nuclear factor 1 antibody Thyroid nuclear factor antibody Thyroid specific enhancer binding protein antibody Thyroid transcription factor 1 antibody Tin man antibody Tinman antibody TITF 1 antibody TITF1 antibody TTF 1 antibody TTF-1 antibody TTF1 antibody |
| Product description | This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AV026640 antibody
BCH antibody
Benign chorea antibody
BHC antibody
Homeobox protein NK 2 homolog A antibody
Homeobox protein NK-2 homolog A antibody
Homeobox protein Nkx 2.1 antibody
Homeobox protein Nkx-2.1 antibody
Homeobox protein Nkx2.1 antibody
NK 2 antibody
NK 2 homolog A antibody
NK2 antibody
NK2 homeobox 1 antibody
NK2 drosophila homolog of A antibody NK2.1 mouse homolog of antibody Nkx 2 1 antibody NKX 2.1 antibody NKX 2A antibody NKX2 1 antibody Nkx2-1 antibody NKX2.1 antibody NKX21_HUMAN antibody NKX2A antibody T EBP antibody T/EBP antibody TEBP antibody Thyroid nuclear factor 1 antibody Thyroid nuclear factor antibody Thyroid specific enhancer binding protein antibody Thyroid transcription factor 1 antibody Tin man antibody Tinman antibody TITF 1 antibody TITF1 antibody TTF 1 antibody TTF-1 antibody TTF1 antibody |
|---|---|
| Molecular Weight(MW) | 103 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q15361 |
|---|
Application
Fig1: Western blot analysis of TTF1 on ?human TTF1 recombinant protein using anti-TTF1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of TTF1 on HEK293 (1) and TTF1-hIgGFc transfected HEK293 (2) cell lysate using anti-TTF1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining TTF1 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:ICC staining TTF1 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-TTF1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-TTF1 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Hela cells with TTF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human TTF1 recombinant protein, TTF1-hIgGFc transfected HEK293 cell lysate, MCF-7, SK-OV-3, Hela, human rectum cancer tissue, human cervical cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
