Application
Fig1: Western blot analysis of CD2 on human CD2 recombinant protein using anti-CD2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CD2 on HEK293 (1) and CD2-hIgGFc transfected HEK293 (2) cell lysate using anti-CD2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CD2 on different cell lysate using anti-CD2 antibody at 1/1,000 dilution. Positive control： Lane1: MOLT4 Lane2: MCF-7 Lane3: Hela Lane4: L1210Application
Fig4:Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD2 antibody. Counter stained with hematoxylin.Application
Fig5:Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with CD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CD2 [B4-D1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 2 antibody CD2 antibody CD2 antigen (p50), sheep red blood cell receptor antibody CD2 antigen antibody CD2 molecule antibody CD2_HUMAN antibody Erythrocyte receptor antibody FLJ46032 antibody LFA-2 antibody LFA-3 receptor antibody LFA2 antibody LFA3 receptor antibody Ly-37 antibody Lymphocyte function antigen 2 antibody lymphocyte-function antigen-2 antibody OTTHUMP00000024366 antibody Rosette receptor antibody Sheep erythrocyte receptor antibody SRBC antibody T cell surface antigen CD2 antibody T-cell surface antigen CD2 antibody T-cell surface antigen T11/Leu-5 antibody T-lymphocyte surface CD2 antigen antibody T11 antibody |
| Product description | CD2 (also designated E-rosette receptor) interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes, as well as on mouse B cells and rat splenic macrophages. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counterreceptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:100-1:500 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | CD 2 antibody
CD2 antibody
CD2 antigen (p50) sheep red blood cell receptor antibody CD2 antigen antibody CD2 molecule antibody CD2_HUMAN antibody Erythrocyte receptor antibody FLJ46032 antibody LFA-2 antibody LFA-3 receptor antibody LFA2 antibody LFA3 receptor antibody Ly-37 antibody Lymphocyte function antigen 2 antibody lymphocyte-function antigen-2 antibody OTTHUMP00000024366 antibody Rosette receptor antibody Sheep erythrocyte receptor antibody SRBC antibody T cell surface antigen CD2 antibody T-cell surface antigen CD2 antibody T-cell surface antigen T11/Leu-5 antibody T-lymphocyte surface CD2 antigen antibody T11 antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Membrane |
| SwissProt ID | P06729 |
|---|
Application
Fig1: Western blot analysis of CD2 on human CD2 recombinant protein using anti-CD2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD2 on HEK293 (1) and CD2-hIgGFc transfected HEK293 (2) cell lysate using anti-CD2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CD2 on different cell lysate using anti-CD2 antibody at 1/1,000 dilution. Positive control： Lane1: MOLT4 Lane2: MCF-7 Lane3: Hela Lane4: L1210
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD2 antibody. Counter stained with hematoxylin.
Application
Fig5:Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with CD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CD2 recombinant protein, CD2-hIgGFc transfected HEK293 cell lysate, MOLT4, MCF-7, Hela, L1210, human lung cancer, human rectum cancer. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:100-1:500 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
