Application
Fig1: Western blot analysis of DAPP1 on human DAPP1 recombinant protein using anti-DAPP1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of DAPP1 on HEK293 (1) and DAPP1-hIgGFc transfected HEK293 (2) cell lysate using anti-DAPP1 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of A549 cells with DAPP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DAPP1 [A9-F7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B cell adapter molecule of 32 kDa antibody B lymphocyte adapter protein Bam32 antibody B-cell adapter molecule of 32 kDa antibody BAM32 antibody DAPP1 antibody DAPP1_HUMAN antibody DKFZp667E0716 antibody Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody hDAPP1 antibody |
| Product description | B cell adapter molecule (BAM32) is also designated dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein. BAM32 is a B cell-associated adapter that is crucial for B cell antigen receptor signaling regulation. BAM32 interacts with Ptdlns and PLC g2 and, upon B cell activation, the protein is phosphorylated on tyrosine residues. It is a mainly cytoplasmic protein that can translocate to the cell membrane after cell stimulation. BAM32, which contains one PH domain and one SH2 domain, is primarily expressed in placenta and lung tissues, but can also be detected in heart, liver, pancreas and brain. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | B cell adapter molecule of 32 kDa antibody B lymphocyte adapter protein Bam32 antibody B-cell adapter molecule of 32 kDa antibody BAM32 antibody DAPP1 antibody DAPP1_HUMAN antibody DKFZp667E0716 antibody Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody hDAPP1 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytoplasm. Membrane |
| SwissProt ID | Q9UN19 |
|---|
Application
Fig1: Western blot analysis of DAPP1 on human DAPP1 recombinant protein using anti-DAPP1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DAPP1 on HEK293 (1) and DAPP1-hIgGFc transfected HEK293 (2) cell lysate using anti-DAPP1 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of A549 cells with DAPP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DAPP1 recombinant protein, DAPP1-hIgGFc transfected HEK293 cell lysate, A549. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
