Application
Fig1: Western blot analysis of DDX58 on human DDX58 recombinant protein using anti-DDX58 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of DDX58 on HEK293 (1) and DDX58-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX58 antibody at 1/1,000 dilution.Application
Fig3: ICC staining DDX58 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:ICC staining DDX58 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5:Flow cytometric analysis of Hela cells with DDX58 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DDX58 [B1-G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ddx58 antibody DDX58_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody DEAD (Asp Glu Ala Asp/His) box polypeptide antibody DEAD box protein 58 antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody DKFZp434J1111 antibody DKFZp686N19181 antibody FLJ13599 antibody Probable ATP dependent RNA helicase DDX58 antibody Probable ATP-dependent RNA helicase DDX58 antibody Retinoic acid inducible gene 1 protein antibody Retinoic acid-inducible gene 1 protein antibody Retinoic acid-inducible gene I protein antibody RIG I antibody Rig-1 antibody RIG-I antibody RIG1 antibody rigi antibody RLR 1 antibody RNA helicase antibody RNA helicase RIG I antibody SGMRT2 antibody |
| Product description | The innate immune system senses viral infection by recognizing many different viral components and triggering specific antiviral responses. Intracellular double-stranded RNA (dsRNA) is a major sign of replication for many viruses. Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN. RIG-I does this by using its helicase domain to bind to viral dsRNA, thus transmitting the activation signal for IFN through IkB kinase-related kinases and inducing IFN expression. RIG-I is expressed in the cytoplasm of fibroblasts and conventional dendritic cells and can distinguish between many different RNA viruses. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Ddx58 antibody DDX58_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody DEAD (Asp Glu Ala Asp/His) box polypeptide antibody DEAD box protein 58 antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody DKFZp434J1111 antibody DKFZp686N19181 antibody FLJ13599 antibody Probable ATP dependent RNA helicase DDX58 antibody Probable ATP-dependent RNA helicase DDX58 antibody Retinoic acid inducible gene 1 protein antibody Retinoic acid-inducible gene 1 protein antibody Retinoic acid-inducible gene I protein antibody RIG I antibody Rig-1 antibody RIG-I antibody RIG1 antibody rigi antibody RLR 1 antibody RNA helicase antibody RNA helicase RIG I antibody SGMRT2 antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | O95786 |
|---|
Application
Fig1: Western blot analysis of DDX58 on human DDX58 recombinant protein using anti-DDX58 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of DDX58 on HEK293 (1) and DDX58-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX58 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining DDX58 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:ICC staining DDX58 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5:Flow cytometric analysis of Hela cells with DDX58 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human DDX58 recombinant protein, DDX58-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
