Application
Fig1: Western blot analysis of TLR9 on human TLR9 recombinant protein using anti-TLR9 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of TLR9 on HEK293 (1) and TLR9-hIgGFc transfected HEK293 (2) cell lysate using anti-TLR9 antibody at 1/1,000 dilution.Application
Fig3: ICC staining TLR9 (green) and Actin filaments (red) in?SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human ?ovarian cancer tissue using anti-TLR9 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer? tissue using anti-TLR9 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of A549 cells with TLR9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | TLR9 [H2-B12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 289 antibody CD289 antibody TLR 9 antibody TLR9 antibody TLR9_HUMAN antibody Toll like receptor 9 antibody Toll like receptor 9 isoform A precursor antibody Toll like receptor 9 isoform B antibody Toll-like receptor 9 antibody |
| Product description | arization in developing Drosophila embryos and participate in host immunity. The TLR family members are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transductions. TLRs are type I transmembrane receptors that contain an extracellular domain consisting of several leucine-rich regions and a single cytoplasmic Toll/IL-1R like domain. Three TLR family members, TLR7, TLR8 and TLR9, belong to a subfamily of TLRs which are differentially expressed. TLR7 is expressed in lung, placenta and spleen. TLR8 is expressed in lung and peripheral blood leukocytes, and TLR9 is predominantly expressed in spleen, lymph nodes, bone marrow and peripheral blood leukocytes. TLR7, TLR8 and TLR9 stimulate the NFκB signaling pathway, suggesting that they play a role in the immune response. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD 289 antibody CD289 antibody TLR 9 antibody TLR9 antibody TLR9_HUMAN antibody Toll like receptor 9 antibody Toll like receptor 9 isoform A precursor antibody Toll like receptor 9 isoform B antibody Toll-like receptor 9 antibody |
|---|---|
| Molecular Weight(MW) | 116 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. Cell membrane |
| SwissProt ID | Q9NR96 |
|---|
Application
Fig1: Western blot analysis of TLR9 on human TLR9 recombinant protein using anti-TLR9 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of TLR9 on HEK293 (1) and TLR9-hIgGFc transfected HEK293 (2) cell lysate using anti-TLR9 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining TLR9 (green) and Actin filaments (red) in?SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human ?ovarian cancer tissue using anti-TLR9 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer? tissue using anti-TLR9 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of A549 cells with TLR9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human TLR9 recombinant protein, TLR9-hIgGFc transfected HEK293 cell lysate, SK-OV-3, A549, human ?ovarian cancer tissue, human endometrial cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
