Application
Fig1: Western blot analysis of KPNA2 on human KPNA2 recombinant protein using anti-KPNA2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of KPNA2 on HEK293 (1) and KPNA2-hIgGFc transfected HEK293 (2) cell lysate using anti-KPNA2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of KPNA2 on different cell lysate using anti-KPNA2 antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: HEK293 Lane3: NIH/3T3Application
Fig4:ICC staining KPNA2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KPNA2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer?tissue using anti-KPNA2 antibody. Counter stained with hematoxylin.Application
Fig7:Flow cytometric analysis of Hela cells with KPNA2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | KPNA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IMA1_HUMAN antibody Importin alpha 1 antibody Importin alpha 2 antibody Importin alpha 2 subunit antibody Importin alpha P1 antibody Importin subunit alpha-1 antibody IPO A1 antibody IPOA 1 antibody IPOA1 antibody Karyopherin alpha 2 antibody Karyopherin alpha 2 RAG cohort 1 antibody Karyopherin alpha 2 subunit antibody Karyopherin subunit alpha-2 antibody KPNA2 antibody KPNA2 protein antibody Pendulin antibody QIP 2 antibody QIP2 antibody RAG cohort 1 antibody RAG cohort protein 1 antibody RCH 1 antibody RCH1 antibody SRP 1 antibody SRP1 alpha antibody SRP1 antibody SRP1-alpha antibody SRP1alpha antibody |
| Product description | Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin a1 and karyopherin β1 subunits. Karyopherin a1 functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin β1 serves as an adapter, tethering the karyopherin α1/substrate complex to docking proteins on the nuclear envelope termed nucleoporins. Karyopherin a2 has been shown to complex with Epstein-Barr virus nuclear antigen 1 (EBNA1). Certain RNA-binding proteins are imported to the nucleus by karyopherin β2, and karyopherin β3 appears to be involved in the import of some ribosomal proteins. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | IMA1_HUMAN antibody Importin alpha 1 antibody Importin alpha 2 antibody Importin alpha 2 subunit antibody Importin alpha P1 antibody Importin subunit alpha-1 antibody IPO A1 antibody IPOA 1 antibody IPOA1 antibody Karyopherin alpha 2 antibody Karyopherin alpha 2 RAG cohort 1 antibody Karyopherin alpha 2 subunit antibody Karyopherin subunit alpha-2 antibody KPNA2 antibody KPNA2 protein antibody Pendulin antibody QIP 2 antibody QIP2 antibody RAG cohort 1 antibody RAG cohort protein 1 antibody RCH 1 antibody RCH1 antibody SRP 1 antibody SRP1 alpha antibody SRP1 antibody SRP1-alpha antibody SRP1alpha antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of KPNA2 on human KPNA2 recombinant protein using anti-KPNA2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of KPNA2 on HEK293 (1) and KPNA2-hIgGFc transfected HEK293 (2) cell lysate using anti-KPNA2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of KPNA2 on different cell lysate using anti-KPNA2 antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: HEK293 Lane3: NIH/3T3
Application
Fig4:ICC staining KPNA2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KPNA2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer?tissue using anti-KPNA2 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of Hela cells with KPNA2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human KPNA2 recombinant protein, KPNA2-hIgGFc transfected HEK293 cell lysate, Hela, HEK293, NIH/3T3, human esophageal cancer? tissue, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
